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Control of the signaling role of PtdIns(4)P at the plasma membrane through H2O2-dependent inactivation of synaptojanin 2 during endocytosis

Title
Control of the signaling role of PtdIns(4)P at the plasma membrane through H2O2-dependent inactivation of synaptojanin 2 during endocytosis
Authors
JoSu InKimSureeLimJung MiRheeSue GooJeongBo-GyeongChaSun-ShinChangJae-ByumKangDongmin
Ewha Authors
강동민김수리
SCOPUS Author ID
강동민scopus
Issue Date
2024
Journal Title
Redox Biology
ISSN
2213-2317JCR Link
Citation
Redox Biology vol. 71
Keywords
Phosphatidylinositol 4-phosphate [PtdIns(4)P]Protein oxidationSynaptojanin (synj)EndocytosisHydrogen peroxide
Publisher
Elsevier B.V.
Indexed
SCIE; SCOPUS scopus
Document Type
Article
Abstract
Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] is implicated in various processes, including hormone-induced signal transduction, endocytosis, and exocytosis in the plasma membrane. However, how H2O2 accumulation regulates the levels of PtdIns(4,5)P2 in the plasma membrane in cells stimulated with epidermal growth factors (EGFs) is not known. We show that a plasma membrane PtdIns(4,5)P2-degrading enzyme, synaptojanin (Synj) phosphatase, is inactivated through oxidation by H2O2. Intriguingly, H2O2 inhibits the 4-phosphatase activity of Synj but not the 5-phosphatase activity. In EGF-activated cells, the oxidation of Synj dual phosphatase is required for the transient increase in the plasma membrane levels of phosphatidylinositol 4-phosphate [PtdIns(4)P], which can control EGF receptor-mediated endocytosis. These results indicate that intracellular H2O2 molecules act as signaling mediators to fine-tune endocytosis by controlling the stability of plasma membrane PtdIns(4)P, an intermediate product of Synj phosphoinositide dual phosphatase. © 2024 The Authors
DOI
10.1016/j.redox.2024.103097
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자연과학대학 > 생명과학전공 > Journal papers
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