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Identification of dendritic cell precursor from the CD11c<SUP>+</SUP> cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand
- Title
- Identification of dendritic cell precursor from the CD11c<SUP>+</SUP> cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand
- Authors
- In, Hyunju; Park, Ji Soo; Shin, Hyun Soo; Ryu, Seul Hye; Sohn, Moah; Choi, Wanho; Park, Sejung; Hwang, Soomin; Park, Jeyun; Che, Lihua; Kim, Tae-Gyun; Chu, Min Kyung; Na, Hye Young; Park, Chae Gyu
- Ewha Authors
- 박채규
- SCOPUS Author ID
- 박채규
- Issue Date
- 2023
- Journal Title
- FRONTIERS IN IMMUNOLOGY
- ISSN
- 1664-3224
- Citation
- FRONTIERS IN IMMUNOLOGY vol. 14
- Keywords
- antigen presentation; bone marrow cells; cell differentiation; cultured cells; dendritic cells; FLT3 ligand; GM-CSF; precursor cells
- Publisher
- FRONTIERS MEDIA SA
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Dendritic cells (DCs) are readily generated from the culture of mouse bone marrow (BM) treated with either granulocyte macrophage-colony stimulating factor (GM-CSF) or FMS-like tyrosine kinase 3 ligand (FLT3L). CD11c(+)MHCII(+) or CD11c(+)MHCII(hi) cells are routinely isolated from those BM cultures and generally used as in vitro-generated DCs for a variety of experiments and therapies. Here, we examined CD11c(+) cells in the BM culture with GM-CSF or FLT3L by staining with a monoclonal antibody 2A1 that is known to recognize mature or activated DCs. Most of the cells within the CD11c(+)MHCII(hi) DC gate were 2A1(+) in the BM culture with GM-CSF (GM-BM culture). In the BM culture with FLT3L (FL-BM culture), almost of all the CD11c(+)MHCII(hi) cells were within the classical DC2 (cDC2) gate. The analysis of FL-BM culture revealed that a majority of cDC2-gated CD11c(+)MHCII(hi) cells exhibited a 2A1(-)CD83(-)CD115(+)CX(3)CR1(+) phenotype, and the others consisted of 2A1(+)CD83(+)CD115(-)CX(3)CR1(- )and 2A1(-)CD83(-)CD115(-)CX(3)CR1(-) cells. According to the antigen uptake and presentation, morphologies, and gene expression profiles, 2A1(-)CD83(-)CD115(-)CX(3)CR1(-) cells were immature cDC2s and 2A1(+)CD83(+)CD115(-)CX(3)CR1(-) cells were mature cDC2s. Unexpectedly, however, 2A1(-)CD83(-)CD115(+)CX(3)CR1(+) cells, the most abundant cDC2-gated MHCII(hi )cell subset in FL-BM culture, were non-DCs. Adoptive cell transfer experiments in the FL-BM culture confirmed that the cDC2-gated MHCIIhi non-DCs were precursors to cDC2s, i.e., MHCIIhi pre-cDC2s. MHCIIhi pre-cDC2s also expressed the higher level of DC-specific transcription factor Zbtb46 as similarly as immature cDC2s. Besides, MHCIIhi pre-cDC2s were generated only from pre-cDCs and common DC progenitor (CDP) cells but not from monocytes and common monocyte progenitor (cMoP) cells, verifying that MHCII(hi )pre-cDC2s are close lineage to cDCs. All in all, our study identified and characterized a new cDC precursor, exhibiting a CD11c(+)MHCII(hi)CD115(+)CX(3)CR1(+) phenotype, in FL-BM culture.
- DOI
- 10.3389/fimmu.2023.1179981
- Appears in Collections:
- 연구기관 > 세포항상성연구센터 > Journal papers
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