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Genetic characterization of tetracycline-resistant Staphylococcus aureus with reduced vancomycin susceptibility using whole-genome sequencing

Title
Genetic characterization of tetracycline-resistant Staphylococcus aureus with reduced vancomycin susceptibility using whole-genome sequencing
Authors
KimYu-HeeParkJunghunChungHae-Sun
Ewha Authors
정혜선김유희
SCOPUS Author ID
정혜선scopus; 김유희scopus
Issue Date
2024
Journal Title
Archives of Microbiology
ISSN
0302-8933JCR Link
Citation
Archives of Microbiology vol. 206, no. 1
Keywords
Cell wall thickeningComparative genomic analysisReduced vancomycin susceptibilityStaphylococcus aureusWhole-genome sequencing
Publisher
Springer Science and Business Media Deutschland GmbH
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
This study aimed to analyze the genetic characteristics of Staphylococcus aureus with reduced vancomycin susceptibility (RVS-SA). Whole-genome sequencing was performed on 27 RVS-SA clinical isolates, and comparative genomic analysis was performed using S. aureus reference strains. Pan-genome orthologous groups (POGs) were identified that were present in RVS-SA but absent in the reference strains, but further analysis showed that the presence of these POGs was influenced by tetracycline resistance rather than vancomycin resistance. Therefore, we restricted our analysis to tetracycline-resistant (tetR) RVS-SA and tetR vancomycin-susceptible S. aureus (VSSA). Phylogenomic analysis showed them to be closely related, and further analysis revealed the presence of an uncharacterized protein SAB0394 and the absence of lytA in tetR RVS-SA, which are involved in cell wall thickening. In summary, using whole-genome sequencing we identified gain or loss of genes in tetR RVS-SA strains. These findings provide insights into the investigation of mechanisms associated with reduced vancomycin susceptibility and have the potential to contribute to the development of molecular biomarkers for the rapid and efficient detection of RVS-SA. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
DOI
10.1007/s00203-023-03760-0
Appears in Collections:
의과대학 > 의학과 > Journal papers
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