NL repository
menu
검색
Library
Browse
Communities & Collections
By Date
Authors
Titles
Subject
My Repository
My Account
Receive email updates
Edit Profile
DSpace at EWHA
의과대학
의학과
Journal papers
View : 133 Download: 0
miR-616-3p alleviates inflammatory response by targeting C-X-C motif chemokine ligand 5
Title
miR-616-3p alleviates inflammatory response by targeting C-X-C motif chemokine ligand 5
Authors
Lee
;
Suyeon
;
Kim
;
Suji
;
Tae-Jin
;
Lim
;
Jae Hyang
;
Woo
;
Chang-Hoon
Ewha Authors
임재향
SCOPUS Author ID
임재향
Issue Date
2024
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291X
Citation
Biochemical and Biophysical Research Communications vol. 691
Keywords
CHOP
;
CXCL5
;
Inflammation
;
Lung epithelial cells
;
miR-616
Publisher
Elsevier B.V.
Indexed
SCIE; SCOPUS
Document Type
Article
Abstract
C/EBP homologous protein (CHOP) is a key regulator in ER stress-mediated signaling pathway via PERK-dependent unfolded protein response. It has been known that microRNA-616 (miR-616) is produced from the intron of the human DDIT3 gene encoding CHOP and increased by ER stress. However, the role of miR-616 and its targets are not fully addressed yet. Here we try to identify a novel target of miR-616 in human lung epithelial cells. Microarray analysis showed that CXCL5 is the most downregulated gene by miR-616 overexpression in A549 cells. We also found that CXCL5 mRNA and protein levels were significantly reduced by miR-616 mimic in the presence or absence of TNFα, while anti-miR-616 enhanced CXCL5 expression. In addition, miR-616-3p targeting sequence in 3′UTR of CXCL5 was confirmed by luciferase reporter assay suggesting that miR-616-3p directly binds to 3′UTR of CXCL5 and inhibits CXCL5 expression. Finally, we confirmed that conditioned medium from A549 cells treated with TNFα or Streptococcus pneumoniae lysates increased intra-alveolar neutrophil infiltration in a mouse model of pulmonary inflammation, while this induction was significantly reduced in a conditioned medium from cells transfected with miR-616-3p. These results suggest that miR-616-3p can alleviate CXCL5-induced pulmonary inflammatory response via targeting 3′UTR of CXCL5 gene. © 2023 Elsevier Inc.
DOI
10.1016/j.bbrc.2023.149335
Appears in Collections:
의과대학
>
의학과
>
Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML
Show full item record
Find@EWHA
트윗하기
BROWSE
Communities & Collections
By Date
Authors
Titles
Subject