Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이대기 | * |
dc.date.accessioned | 2023-10-23T16:30:43Z | - |
dc.date.available | 2023-10-23T16:30:43Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 2352-345X | * |
dc.identifier.other | OAK-33935 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/266421 | - |
dc.description.abstract | BACKGROUND & AIMS: Fibrosis development in ulcerative colitis is associated directly with the severity of mucosal inflammation, which increases the risk of colorectal cancer. The transforming growth factor -5 (TGF-5) signaling pathway is an important source of tissue fibrogenesis, which is stim-ulated directly by reactive oxygen species produced from nicotinamide adenine dinucleotide phosphate oxidases (NOX). Among members of the NOX family, NOX4 expression is up -regulated in patients with fibrostenotic Crohn's disease (CD) and in dextran sulfate sodium (DSS)-induced murine colitis. The aim of this study was to determine whether NOX4 plays a role in fibrogenesis during inflammation in the colon using a mouse model.METHODS: Acute and recovery models of colonic inflamma-tion were performed by DSS administration to newly gener-ated Nox4-/-mice. Pathologic analysis of colon tissues was performed, including detection of immune cells, proliferation, and fibrotic and inflammatory markers. RNA sequencing was performed to detect differentially expressed genes between Nox4-/-and wild-type mice in both the untreated and DSS-treated conditions, followed by functional enrichment anal-ysis to explore the molecular mechanisms contributing to pathologic differences during DSS-induced colitis and after recovery.RESULTS: Nox4-/-mice showed increased endogenous TGF-5 signaling in the colon, increased reactive oxygen species levels, intensive inflammation, and an increased fibrotic region after DSS treatment compared with wild-type mice. Bulk RNA sequencing confirmed involvement of canonical TGF-5 signaling in fibrogenesis of the DSS-induced colitis model. Up -regulation of TGF-5 signaling affects collagen activation and T-cell lineage commitment, increasing the susceptibility for inflammation.CONCLUSIONS: Nox4 protects against injury and plays a crucial role in fibrogenesis in DSS-induced colitis through canonical TGF-5 signaling regulation, highlighting a new treatment target. (Cell Mol Gastroenterol Hepatol 2023 | * |
dc.language | English | * |
dc.publisher | ELSEVIER INC | * |
dc.subject | Fibrostenotic CD | * |
dc.subject | T-Cell Lineage Commitment | * |
dc.subject | RNA-Sequencing | * |
dc.subject | Oxidative Stress | * |
dc.title | Role of Nox4 in Mitigating Inflammation and Fibrosis in Dextran Sulfate Sodium-Induced Colitis | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 16 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 411 | * |
dc.relation.lastpage | 429 | * |
dc.relation.journaltitle | CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY | * |
dc.identifier.doi | 10.1016/j.jcmgh.2023.05.002 | * |
dc.identifier.wosid | WOS:001049924600001 | * |
dc.author.google | Lee, Yura | * |
dc.author.google | Kim, Sung-Hee | * |
dc.author.google | Jeong, Haengdueng | * |
dc.author.google | Kim, Kwang H. | * |
dc.author.google | Jeon, Donghun | * |
dc.author.google | Cho, Yejin | * |
dc.author.google | Lee, Daekee | * |
dc.author.google | Nam, Ki Taek | * |
dc.contributor.scopusid | 이대기(37047040400) | * |
dc.date.modifydate | 20231120165418 | * |