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약학대학
약학과
Journal papers
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Peroxiredoxin V Protects against UVB-Induced Damage of Keratinocytes
Title
Peroxiredoxin V Protects against UVB-Induced Damage of Keratinocytes
Authors
Kim S.R.
;
Park J.W.
;
Lee B.-H.
;
Lim K.M.
;
Chang T.-S.
Ewha Authors
임경민
SCOPUS Author ID
임경민
Issue Date
2023
Journal Title
Antioxidants
ISSN
2076-3921
Citation
Antioxidants vol. 12, no. 7
Keywords
apoptosis
;
keratinocytes
;
mitochondrial dysfunction
;
peroxiredoxin V
;
reactive oxygen species
;
ultraviolet B irradiation
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Indexed
SCIE; SCOPUS
Document Type
Article
Abstract
Ultraviolet B (UVB) irradiation generates reactive oxygen species (ROS), which can damage exposed skin cells. Mitochondria and NADPH oxidase are the two principal producers of ROS in UVB-irradiated keratinocytes. Peroxiredoxin V (PrxV) is a mitochondrial and cytosolic cysteine-dependent peroxidase enzyme that robustly removes H2O2. We investigated PrxV’s role in protecting epidermal keratinocytes against UVB-induced ROS damage. We separated mitochondrial and cytosolic H2O2 levels from other types of ROS using fluorescent H2O2 indicators. Upon UVB irradiation, PrxV-knockdown HaCaT human keratinocytes showed higher levels of mitochondrial and cytosolic H2O2 than PrxV-expressing controls. PrxV depletion enhanced hyperoxidation-mediated inactivation of mitochondrial PrxIII and cytosolic PrxI and PrxII in UVB-irradiated keratinocytes. PrxV-depleted keratinocytes exhibited mitochondrial dysfunction and were more susceptible to apoptosis through decreased oxygen consumption rate, loss of mitochondrial membrane potential, cardiolipin oxidation, cytochrome C release, and caspase activation. Our findings show that PrxV serves to protect epidermal keratinocytes from UVB-induced damage such as mitochondrial dysfunction and apoptosis, not only by directly removing mitochondrial and cytosolic H2O2 but also by indirectly improving the catalytic activity of mitochondrial PrxIII and cytosolic PrxI and PrxII. It is possible that strengthening PrxV defenses could aid in preventing UVB-induced skin damage. © 2023 by the authors.
DOI
10.3390/antiox12071435
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