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CUDC-907 suppresses epithelial-mesenchymal transition, migration and invasion in a 3D spheroid model of bladder cancer
- Title
- CUDC-907 suppresses epithelial-mesenchymal transition, migration and invasion in a 3D spheroid model of bladder cancer
- Authors
- Ho, Jin-Nyoung; Jeon, Jessie Sungyun; Kim, Dan Hyo; Ryu, Hoyoung; Lee, Sangchul
- Ewha Authors
- 류호영
- SCOPUS Author ID
- 류호영
- Issue Date
- 2023
- Journal Title
- ONCOLOGY REPORTS
- ISSN
- 1021-335X
1791-2431
- Citation
- ONCOLOGY REPORTS vol. 49, no. 6
- Keywords
- bladder cancer spheroid; CUDC-907; epithelial-mesenchymal transition; migration; invasion
- Publisher
- SPANDIDOS PUBL LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- CUDC-907 is a novel inhibitor of phosphoinositide 3-kinase and histone deacetylase. It exerts anticancer activities by inducing apoptosis and inhibiting the growth and metastases of various tumors. However, the anticancer effects of CUDC-907 on bladder cancer have not been previously reported. Thus, the present study aimed to examine the anticancer effects of CUDC-907 on 2D monolayer and 3D spheroid models of T24 cells established from highly malignant human grade III urinary bladder carcinoma and cisplatin-resistant T24R2 cells generated by 17 months of exposure to cisplatin, starting at 0.01 mu g/ml and increasing stepwise to 2 mu g/ml. CUDC-907 treatment significantly reduced the cell viabilities of the monolayer and spheroid cultures in a concentration-dependent manner. The IC50 value of CUDC-907 was higher in the bladder cancer spheroids than in the monolayers. Treatment with CUDC-907 suppressed epithelial-mesenchymal transition via decreasing vimentin and E-cadherin and consequently inhibited the migration and invasion of the bladder cancer spheroids. In addition, it promoted apoptosis and increased the expression of apoptosis-related genes, such as Bax and caspases. In conclusion, CUDC-907 exerted anticancer effects by reducing the viability, migration and invasion, and inducing apoptosis of bladder cancer spheroids. These results suggest that CUDC-907 is a potent agent for the treatment of bladder cancer.
- DOI
- 10.3892/or.2023.8567|http://dx.doi.org/10.3892/or.2023.8567
- Appears in Collections:
- 의료원 > 의료원 > Journal papers
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