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Lyso-globotriaosylsphingosine induces endothelial dysfunction via autophagy-dependent regulation of necroptosis
- Title
- Lyso-globotriaosylsphingosine induces endothelial dysfunction via autophagy-dependent regulation of necroptosis
- Authors
- Hwang A.-R.; Park S.; Woo C.-H.
- Ewha Authors
- 박성희
- SCOPUS Author ID
- 박성희
- Issue Date
- 2023
- Journal Title
- Korean Journal of Physiology and Pharmacology
- ISSN
- 1226-4512
- Citation
- Korean Journal of Physiology and Pharmacology vol. 27, no. 3, pp. 231 - 240
- Keywords
- Autophagy; Cellular senescence; Glycosphingolipids; Inflammation; Necroptosis
- Publisher
- Korean Physiological Soc. and Korean Soc. of Pharmacology
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Fabry disease is a lysosomal storage disorder characterized by the lysosomal accumulations of glycosphingolipids in a variety of cytotypes, which include endothelial cells. The disease is inherited and originates from an error in glycosphingolipid catabolism caused by insufficient α-galactosidase A activity, which causes uncontrolled progressive storage of intracellular globotriaosylceramide (Gb3) in the vasculature and extracellular accumulation of lyso-Gb3 (a deacetylated soluble form of Gb3). Necrosis can lead to inflammation, which exacerbates necrosis and creates a positive feedback loop that triggers necroinflammation. However, the role played by necroptosis, a form of programmed necrotic cell death, in the cell-to-cell inflammatory reaction between epithelial and endothelial cells is unclear. Thus, the present study was undertaken to determine whether lyso-Gb3 induces necroptosis and whether necroptosis inhibition protects endothelial dysfunction against lyso-Gb3 inflamed retinal pigment epithelial cells. We found lyso-Gb3 induced necroptosis of a retinal pigment epithelial cell line (ARPE-19) in an autophagy-dependent manner and that conditioned media (CM) from ARPE-19 cells treated with lyso-Gb3 induced the necroptosis, inflammation, and senescence of human umbilical vein endothelial cells. In addition, a pharmacological study showed CM from lyso-Gb3 treated ARPE-19 cells induced endothelial necroptosis, inflammation, and senescence were significantly inhibited by an autophagy inhibitor (3-MA) and by two necroptosis inhibitors (necrostatin and GSK-872), respectively. These results demonstrate lyso-Gb3 induces necroptosis via autophagy and suggest that lyso-Gb3 inflamed retinal pigment epithelial cells trigger endothelial dysfunction via the autophagy-dependent necroptosis pathway. This study suggests the involvement of a novel autophagy-dependent necroptosis pathway in the regulation of endothelial dysfunction in Fabry disease. © 2023 Korean Physiological Soc. and Korean Soc. of Pharmacology. All rights reserved.
- DOI
- 10.4196/KJPP.2023.27.3.231
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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