The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srI kappa Bs) in Chronic Post-Ischemia Pain (CPIP) Models

Abstract

Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NF kappa B) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srI kappa B, I kappa B; inhibitor of NF kappa B) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srI kappa B on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srI kappa B, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srI kappa B increased PWT compared to the vehicle and pregabalin, and the relative densities of p-I kappa B and I kappa B showed significant changes compared to the vehicle 24 h after Exo-srI kappa B injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srI kappa B in mice with CPIP. In conclusion, our results showed more specifically the role of NF kappa B in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.