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Noggin-mediated effects on metabolite profiles of microglia and oligodendrocytes after ischemic insult

Title
Noggin-mediated effects on metabolite profiles of microglia and oligodendrocytes after ischemic insult
Authors
Lee J.Shin J.A.Lee E.-M.Nam M.Park E.-M.
Ewha Authors
박은미
SCOPUS Author ID
박은미scopus
Issue Date
2023
Journal Title
Journal of pharmaceutical and biomedical analysis
ISSN
1873-264XJCR Link
Citation
Journal of pharmaceutical and biomedical analysis vol. 224, pp. 115196
Keywords
GlycerolGlycerol-3-phosphate dehydrogenase 1Ischemic strokeMetabolitesMyelinationNoggin
Publisher
NLM (Medline)
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Recent studies show that shifts in energy metabolism in activated microglia are linked to their functions and immune responses in the ischemic brain. We previously reported that an antagonist of the bone morphogenetic protein, noggin, enhanced myelination in the ischemic brain during the chronic phase, and conditioned media (CM) from activated BV2 microglia treated with noggin after ischemia/reperfusion (I/R) increased the expression of myelin basic protein (MBP) in oligodendrocytes (MO3.13). To determine whether noggin induced changes in cell metabolism, metabolite profiles in BV2 and MO3.13 cells were analyzed by untargeted metabolomics using 1H nuclear magnetic resonance spectroscopy. Compared to vehicle-treated BV2 cells, noggin treatment (100 ng/mL for 3 h after I/R) suppressed the I/R-induced increase in intracellular glucose and lactate levels but increased extracellular levels of glucose and several amino acids. When MO3.13 cells were exposed to noggin CM from BV2 cells, most of the vehicle CM-induced changes in the levels of metabolites such as choline, formate, and intermediates of oxidative phosphorylation were reversed, while the glycerol level was markedly increased. An increase in glycerol level was also observed in the noggin-treated ischemic brain and was further supported by the expression of glycerol-3-phosphate dehydrogenase 1 (required for glycerol synthesis) in the cytoplasm of MBP-positive oligodendrocytes in the ischemic brains treated with noggin. These results suggest that noggin-induced changes in the metabolism of microglia provide a favorable environment for myelin synthesis in oligodendrocytes during the recovery phase after ischemic stroke. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
DOI
10.1016/j.jpba.2022.115196
Appears in Collections:
의과대학 > 의학과 > Journal papers
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