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Protein transduction domain of translationally controlled tumor protein: characterization and application in drug delivery
- Title
- Protein transduction domain of translationally controlled tumor protein: characterization and application in drug delivery
- Authors
- Maeng, Jeehye; Lee, Kyunglim
- Ewha Authors
- 이경림; 맹지혜
- SCOPUS Author ID
- 이경림; 맹지혜
- Issue Date
- 2022
- Journal Title
- DRUG DELIVERY
- ISSN
- 1071-7544
1521-0464
- Citation
- DRUG DELIVERY vol. 29, no. 1, pp. 3009 - 3021
- Keywords
- Cell-penetrating peptide; drug delivery; protein transduction domain; translationally controlled tumor protein; translocation mechanism
- Publisher
- TAYLOR &
FRANCIS LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Our research group reported in 2011 the discovery of a novel cell-penetrating moiety in the N-terminus of the human translationally controlled tumor protein (TCTP). This moiety was responsible for the previously noted membrane translocating ability of purified full-length TCTP. The hydrophobic nature of TCTP-derived protein transduction domain (TCTP-PTD) endowed it with unique characteristics compared to other well-known cationic PTDs, such as TAT-PTD. TCTP-PTD internalizes partly through lipid-raft/caveolae-dependent endocytosis and partly by macropinocytosis. After cell entry, caveosome-laden TCTP-PTD appears to move to the cytoplasm and cytoskeleton except for the nucleus possibly through the movement to endoplasmic reticulum (ER). TCTP-PTD efficiently facilitates delivery of various types of cargos, such as peptides, proteins, and nucleic acids in vitro and in vivo. It is noteworthy that TCTP-PTD and its variants promote intranasal delivery of antidiabetics including, insulin and exendin-4 and of antigens for immunization in vivo, suggesting its potential for drug delivery. In this review, we attempted to describe recent advances in the understanding regarding the identification of TCTP-PTD, the characteristics of its cellular uptake, and the usefulness as a vehicle for delivery into cells of a variety of drugs and macromolecules. Our investigative efforts are continuing further to delineate the details of the functions and the regulatory mechanisms of TCTP-PTD-mediated cellular penetration and posttranslational modification of TCTP in physiologic and pathological processes. This is a review of what we currently know regarding TCTP-PTD and its use as a vehicle for the transduction of drugs and other molecules.
- DOI
- 10.1080/10717544.2022.2122636
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
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