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C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes

Title
C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes
Authors
Schubert, ChristopherKim, Nam YeunUnden, GottfriedBin Kim, Ok
Ewha Authors
김옥빈김남연
SCOPUS Author ID
김옥빈scopus
Issue Date
2022
Journal Title
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097JCR Link

1574-6968JCR Link
Citation
FEMS MICROBIOLOGY LETTERS vol. 369, no. 1
Keywords
metabolonC-4-dicarboxylate transporterL-aspartateEscherichia coliBACTHmSPINE
Publisher
OXFORD UNIV PRESS
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Metabolons represent the structural organization of proteins for metabolic or regulatory pathways. Here, the interaction of fumarase FumB, aspartase AspA, and L-tartrate dehydratase TtdAB with the C4-dicarboxylate (C4-DC) transporters DcuA, DcuB, DcuC, and the L-tartrate transporter TtdT of Escherichia coli was tested by a bacterial two-hybrid (BACTH) assay in situ, or by co-chromatography using mSPINE (membrane Streptavidin protein interaction experiment). From the general C4-DC transporters, DcuB interacted with FumB and AspA, DcuA with AspA, whereas DcuC interacted with neither FumB nor AspA. Moreover, TtdT did not interact with TtdAB. The fumB-dcuB, the dcuA-aspA, and the ttdAB-ttdT genes encoding the respective proteins colocalize on the genome and each pair of genes forms cotranscripts, whereas the dcuC gene lies alone. The data suggest the formation of DcuB/FumB and DcuB/AspA metabolons for the uptake of L-malate, or L-aspartate, and their conversion to fumarate for fumarate respiration and excretion of the product succinate. The DcuA/AspA metabolon catalyzes uptake and conversion of L-aspartate to fumarate coupled to succinate excretion. The DcuA/AspA metabolon provides ammonia at the same time for nitrogen assimilation (ammonia shuttle). On the other hand, TtdT and TtdAB are not organized in a metabolon. Reasons for the formation (DcuA/AspA, DcuB/FumB, and DcuB/AspA) or nonformation (DcuC, TtdT, and TtdAB) of metabolons are discussed based on their metabolic roles. Experimental measurement for spatial proximity revealed that transporters for general C-4-dicarboxylates, such as L-malate and L-aspartate, form metabolons with cytosolic enzymes, however, no metabolons were observed for noncommon C-4-dicarboxylates.
DOI
10.1093/femsle/fnac078
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자연과학대학 > 생명과학전공 > Journal papers
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