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How Significant Are Xpert Xpress SARS-CoV-2 Test Findings When Only an N2 Gene Is Detected?

Title
How Significant Are Xpert Xpress SARS-CoV-2 Test Findings When Only an N2 Gene Is Detected?
Authors
So, Min-KyungChung, Hae-SunLee, Duk HeeLee, Miae
Ewha Authors
이미애정혜선이덕희소민경
SCOPUS Author ID
이미애scopus; 정혜선scopus; 이덕희scopus; 소민경scopus
Issue Date
2022
Journal Title
DIAGNOSTICS
ISSN
2075-4418JCR Link
Citation
DIAGNOSTICS vol. 12, no. 9
Keywords
COVID-19emergency medicinerapid screeningqRT-PCRSARS-CoV-2Xpert
Publisher
MDPI
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The rapid identification of patients infected with COVID-19 during the SARS-CoV-2 pandemic is critical to operating emergency rooms effectively. Xpert Xpress SARS-CoV-2 (Xpert) assays are increasingly being used in the rapid screening of COVID-19. We evaluated the clinical performance of Xpert by comparing findings with those of qRT-PCR evaluations and included the clinical features of patients visiting the emergency department. Positive results with Xpert testing (n = 370) were compared with qRT-PCR findings, demonstrating a 91.9% intertest agreement. We reviewed the subsequent COVID-19 test results and SARS-CoV-2 infection histories for individuals showing discrepancies in Xpert and qRT-PCR testing and determined whether the findings were truepositive or false-positive. The true-positive rate for Xpert testing was 95.4% (353/370); the remaining 17 samples (4.6%) were false-positive. All false-positive data for Xpert testing showed N2 signals amplified to Ct values of >= 40 with no E gene signals. Rapid Xpert testing is highly sensitive and shows a good performance overall in challenging situations, such as an emergency room. However, we considered the possibility of false-positive Xpert results given an N2 gene signal only, especially given high Ct values. We recommend interpreting test data with caution and considering retesting over time.
DOI
10.3390/diagnostics12092133
Appears in Collections:
의과대학 > 의학과 > Journal papers
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