Alkasite와 기존의 글라스아이오노머의 무기화학적 특성 비교

Abstract

이 연구의 목적은 최근 개발된 수산화이온 방출 alkasite 수복재와 기존의 글라스아이오노머 시멘트의 pH4.01 산성 탈 이온수에서 장기간 보관 시 무기이온 유리 양상과 pH 변화를 비교하는 것이다. 실험에 사용된 재료는 Cention N (Ivoclar Vivadent, Schaan, Liechtenstein, CN), Fuji II(GC Co, Tokyo, Japan, FII), Ketac™Molar Aplicap™ (3M ESPE, St. Paul, USA, KM)이다. 무기 화학적 성질 평가를 위해 이온 유리량과 pH 변화를 실험하였다(n = 60). Ca, P, Si, Al, Na 이온은 시료의 1mL를 불산 전처리 과정을 거쳐 유도 결합 플라즈마 광학 방출 분광법 (Inductively Coupled Plasma-Optical Emission Spectrometor, 5110, Agilent)을 사용하여 측정하였다. F 이온은 시료의 1mL와 TI-SAB II(Total Ionic Strength Adjusting Buffer, Orion, USA) 1mL를 혼합하여 측정하였다. pH 변화는 경화된 시편을 pH 4.01 탈 이온수 용액(pH4.01 Buffer, 910104, Orion, USA)에 침전시켜 각각 시편의 pH 변화를 측정하였다. 모든 실험 결과는 SPSS Ver 25.0(SPSS Inc, Chicago, IL, U.S.A.)프로그램을 사용하여, 일원 분산 분석(One-Way ANOVA)을 사용하여 분석되었으며, Tukey’s 사후 검정을 실시하였다. 유의 수준 5%로 설정하였다. Ca 이온 유리량은 CN군(235.78±24.33mg/L), KM군(68.15±6.48mg/L), FII군(5.53±4.34 mg/L)순으로 높은 값을 보였다(p<0.05). P 이온 유리량은 CN군(15.49±1.48 mg/L), KM군(5.83±1.09mg/L), FII군(4.62±0.76mg/L)순으로 높게 관찰되었다(p <0.05). Si 이온 유리량은 FII군(78.66±6.43mg/L), CN군(74.96±2.09mg/L), KM군(63.67±8.45mg/L)순으로 높게 나타났다(p<0.05). Al 이온 유리량은 FII군(104.92±14.47mg/L), KM군(62.89±4.35mg/L), CN군(48.11±6.5mg/L순으로 높게 측정하였다(p <0.05). Na 이온 유리량은 KM군(302.29±8.09mg/L), FII군(171.79±34.2mg/L), CN군(73.16±50.54mg/L)순으로 높은 값을 보였다(p<0.05). F 이온 유리량은 FII군(59.01±0.092mg/L), CN군(57.02±0.05mg/L), KM군(27.3±0.05mg/L)순으로 나타났다(p<0.05). pH 변화는 CN군(pH4.65±0.005), FII군(pH4.51±0.008), KM군(pH4.46±0.004)순으로 증가하였다(p<0.05). 이온방출 수복재(ILM) CN과 기존의 글라스아이오노머 시멘트인 FII, KM의 무기 이온 유리양상을 비교하였을 때, CN는 FII, KM보다 Ca, P 이온을 높게 방출하고 pH가 높다. 이는 산성환경에서 Ca와 OH-의 알칼리성 반응을 통해 수산화이온 작용으로 pH가 상승한 것으로 보이며 구강 타액 pH 완충 조절에 기여할 수 있을 것이고, F 이온을 방출하여 항우식 예방효과를 기대할 수 있을 것이다. ;The aim of this study was to compare the inorganic ion release and pH change of a recently developed hydroxide ion-releasing alkasite restoration material and conventional glass ionomer cement in pH 4.01 acidic deionized water for long-term storage. The materials used in the experiment were Cention N (Ivoclar Vivadent, Schaan, Liechtenstein, CN), Fuji II (GC Co, Tokyo, Japan, FII), Ketac ™ Molar Aplicap ™ (3M ESPE, St. Paul, USA, KM To evaluate inorganic chemical properties, the ions release and changes in pH were tested (n=60). The Ca, P, Si, Al, and Na ions were measured using an Inductively Coupled Plasma-Optical Emission Spectrometer (5110, Agilent) through a hydrofluoric acid pretreatment process. F ions were measured by mixing 1 mL of the sample with 1 mL of TI-SAB II (Total Ionic Strength Adjusting Buffer, Orion, USA). The pH change was determined by precipitating the hardened sample in a pH 4.01 deionized water solution (pH 4.01 Buffer, 910104, Orion, USA). All experimental results were analyzed using One-way ANOVA and post hoc test was performed with Tukey's test. The significance level was set at 5%. The Ca ion release was higher in order of CN group (2355.78±24.33mg/L), KM group (68.15±6.48mg/L), and FII group (5.53±4.34mg/L) (p<0.05). The amount of P ion release was observed to be higher in order of CN group (15.49±1.48 mg/L), KM group (5.83±1.09mg/L), and FII group (4.62±0.76mg/L) (P<0.05). The amount of Si ion release was higher in the order of FII group (78.66±6.43mg/L), CN group (74.96±2.09mg/L), and KM group (63.67±8.45mg/L) (p<0.05). The amount of Al ion release was measured higher in the order of FII group (104.92±14.47mg/L), KM group (62.89±4.35 mg/L), and CN group (48.11±6.5 mg/L) (P<0.05). The amount of Na ion release was higher in order of KM group (302.29±8.09mg/L), FII group (171.79±34.2mg/L), and CN group (73.16±50.54 mg/L) (p<0.05). F ion release amount appeared in the order of FII group (59.01±0.092 mg/L), CN group (57.02±0.05mg/L), and KM group (27.3±0.05mg/L) (p<0.05). The change in pH increased in the order of CN group (pH 4.65±0.005), FII group (pH 4.51±0.008), and KM group (pH 4.46±0.004) (p<0.05). When comparing the inorganic ion glass aspect of the Ion-leaching restorative materials (ILM) CN and the conventional glass ionomers FII and KM, CN releases Ca and P ions higher than FII and KM. It is considered that Ca and OH- ions show an alkaline reaction and become hydroxide ions, and the pH rises. Therefore, the hydroxide ions of the restoration material may contribute to the regulation of oral saliva pH buffering. FII releases F ions higher than CN and KM and can be expected to have an anti-cariogenic material.