Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2022-06-02T16:31:03Z | - |
dc.date.available | 2022-06-02T16:31:03Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 0960-8524 | * |
dc.identifier.other | OAK-31471 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/261192 | - |
dc.description.abstract | Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC genes under the promoter Ptac in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester. © 2022 The Authors | * |
dc.language | English | * |
dc.publisher | Elsevier Ltd | * |
dc.subject | 2-pyrone-4 | * |
dc.subject | 6-dicarboxylic acid | * |
dc.subject | Bio-based polyester | * |
dc.subject | Biological funneling | * |
dc.subject | Lignin | * |
dc.subject | Pseudomonas putida | * |
dc.title | Microbial production of 2-pyrone-4,6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putida and its application for the synthesis of bio-based polyester | * |
dc.type | Article | * |
dc.relation.volume | 352 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Bioresource Technology | * |
dc.identifier.doi | 10.1016/j.biortech.2022.127106 | * |
dc.identifier.wosid | WOS:000793757300011 | * |
dc.identifier.scopusid | 2-s2.0-85127811784 | * |
dc.author.google | Lee S. | * |
dc.author.google | Jung Y.J. | * |
dc.author.google | Park S.J. | * |
dc.author.google | Ryu M.-H. | * |
dc.author.google | Kim J.E. | * |
dc.author.google | Song H.M. | * |
dc.author.google | Kang K.H. | * |
dc.author.google | Song B.K. | * |
dc.author.google | Sung B.H. | * |
dc.author.google | Kim Y.H. | * |
dc.author.google | Kim H.T. | * |
dc.author.google | Joo J.C. | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |