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Development of an Electrochemical Dual H2S/Ca2+ Microsensor and Its In Vivo Application to a Rat Seizure Model
- Title
- Development of an Electrochemical Dual H2S/Ca2+ Microsensor and Its In Vivo Application to a Rat Seizure Model
- Authors
- Lee, Jaeyoung; Jeong, Yoonyi; Park, Subin; Suh, Minah; Lee, Youngmi
- Ewha Authors
- 이영미
- SCOPUS Author ID
- 이영미
- Issue Date
- 2021
- Journal Title
- ACS SENSORS
- ISSN
- 2379-3694
- Citation
- ACS SENSORS vol. 6, no. 11, pp. 4089 - 4097
- Keywords
- dual electrochemical microsensor; H2S; Ca2+; seizure; in vivo simultaneous measurement
- Publisher
- AMER CHEMICAL SOC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A dual electrochemical microsensor was fabricated for concurrent monitoring of hydrogen sulfide (H2S) and calcium ions (Ca2+), which are closely linked important signaling species involved in various physiological processes. The dual sensor was prepared using a dual recessed electrode consisting of two platinum (Pt) microdisks (50 mu m in diameter). Each electrode was individually optimized for the best sensing ability toward a target analyte. One electrode (WE1, amperometric H2S sensor) was modified with electrodeposition of Au and electropolymerized polyaniline coating. The other electrode (WE2, all-solid-state Ca(2+)selective electrode) was composed of Ag/AgCl onto the recessed Pt disk formed via electrodeposition/chloridation, followed by silanization and Ca2+-selective membrane loading. The current of WE1 and the potential of WE2 in a dual sensor responded linearly to H2S concentration and logarithm of Ca2+ concentration, respectively, without a crosstalk between the sensing signals. Both WE1 and WE2 presented excellent sensitivity, selectivity (log KH2S,(Amp)(i) <== -3.5, i = CO, NO, O-2, NO2-, AP, AA, DA, and GABA; and log K-Ca2+(pot), <= -3.2 j = Na+, K+, and Mg2+), and fast response time with reasonable stability (during ca. 6 h in vivo experiment). Particularly, WE2 prepared using a mixture of two ionophores (ETH1001 and ETH129) and two plasticizers (2-nitrophenyl octyl ether and bis(2-ethylhexyl) sebacate) showed a very shortened response time (t(R) to attain the Delta E/Delta t slope of 0.6 mV/min = 3.0 +/- 0.2 s, n >= 10), a critically required factor for real-time analysis. The developed sensor was utilized for simultaneous real-time monitoring of H2S and Ca2+ changes at the brain cortex surface of a living rat during spontaneous epileptic seizures induced by a cortical 4-aminopyridine injection. The dynamic changes of H2S and Ca2+ were clearly observed in an intimate correlation with the electrophysiological recording of seizures, demonstrating the sensor feasibility of in vivo and real-time simultaneous measurements of H2S and Ca2+.
- DOI
- 10.1021/acssensors.1c01612
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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