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Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads

Title
Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
Authors
Kim H.Y.Seok J.M.Jung S.Y.Lee M.J.Tran A.N.-T.Yeo S.J.Park S.A.Kim H.S.
Ewha Authors
김한수정수연
SCOPUS Author ID
김한수scopus; 정수연scopus
Issue Date
2022
Journal Title
Biomedicines
ISSN
2227-9059JCR Link
Citation
Biomedicines vol. 10, no. 2
Keywords
AlginateHypoparathyroidismMicrobeadsParathyroid hormoneTonsil-derived mesenchymal stem cells
Publisher
MDPI
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Hypoparathyroidism is an endocrine disorder that occurs because of the inability to produce parathyroid hormone (PTH) effectively. Previously, we reported the efficacy of tonsil-derived mesenchymal stem cells (TMSCs) differentiated into parathyroid-like cells for the treatment of hypoparathyroidism. Here, we investigated the feasibility of three-dimensional structural microbeads fabricated with TMSCs and alginate, a natural biodegradable polymer, to treat hypoparathyroidism. Alginate microbeads were fabricated by dropping a 2% (w/v) alginate solution containing TMSCs into a 5% CaCl2 solution and then differentiated into parathyroid-like cells using activin A and sonic hedgehog for 7 days. The protein expression of PTH, a specific marker of the parathyroid gland, was significantly higher in differentiated alginate microbeads with TMSCs (Al-dT) compared with in undifferentiated alginate microbeads with TMSCs. For in vivo experiments, we created the hypoparathyroidism animal model by parathyroidectomy (PTX) and implanted alginate microbeads in the dorsal interscapular region. The PTX rats with Al-dT (PTX+Al-dT) showed the highest survival rate and weight change and a gradual increase in serum intact PTH levels. We also detected a higher expression of PTH in retrieved tissues of PTX+Al-dT using immunofluorescence analysis. This study demonstrates that alginate microbeads are potential a new tool as a surgically scalable therapy for treating hypoparathyroidism. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
DOI
10.3390/biomedicines10020440
Appears in Collections:
의과대학 > 의학과 > Journal papers
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