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Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO− in living cells

Title
Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO− in living cells
Authors
Chung J.Kim H.Li H.Yoon J.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2021
Journal Title
Dyes and Pigments
ISSN
0143-7208JCR Link
Citation
Dyes and Pigments vol. 195
Keywords
Cell imagingNIR Fluorescent probepKa regulationReactive nitrogen species
Publisher
Elsevier Ltd
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Peroxynitrite (ONOO−), a reactive nitrogen species (RNS), engages in various cellular processes including inflammation, apoptosis, and necrosis. Therefore, the development of a powerful tool for imaging endogenous ONOO− is important with regard to revealing its underlying physiological or pathological functions. In this work, we introduced dihalogen (Cl, Br, and I) at the ortho-positions of the hydroxyl group of a dicyanoisophorone skeleton to adjust its pKa to be more suitable for bio-imaging in the physiological environments with a high signal-to-noise ratio. Based on the optimized spectral results and the requirement of biocompatibility, the fluorescent probe NIR-dCl was successfully designed and fabricated for the detection of ONOO−. Upon the addition of ONOO− in solution, an obvious NIR fluorescent signal at 680 nm was observed. Moreover, the probe NIR-dCl exhibited high selectivity for sensing ONOO−. Cell imaging results confirmed that probe NIR-dCl was capable of imaging exogenous and endogenous ONOO− in live cells. Thus, we believe that probe NIR-dCl could be an effective tool for studying biochemical processes and ONOO−-related diseases. © 2021 Elsevier Ltd
DOI
10.1016/j.dyepig.2021.109665
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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