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Time-sequential change in immune-related gene expression after irradiation in glioblastoma: next-generation sequencing analysis
- Title
- Time-sequential change in immune-related gene expression after irradiation in glioblastoma: next-generation sequencing analysis
- Authors
- Kim, Yi-Jun; Kim, Kwangsoo; Seo, Soo Yeon; Yu, Juyeon; Kim, Il Han; Kim, Hak Jae; Park, Chul-Kee; Lee, Kye Hwa; Choi, Junjeong; Song, Myung Seon; Kim, Jin Ho
- Ewha Authors
- 김이준
- SCOPUS Author ID
- 김이준
- Issue Date
- 2021
- Journal Title
- ANIMAL CELLS AND SYSTEMS
- ISSN
- 1976-8354
2151-2485
- Citation
- ANIMAL CELLS AND SYSTEMS vol. 25, no. 4, pp. 245 - 254
- Keywords
- Glioblastoma; Radiotherapy; Immune-related signals; Next-generation sequencing; Transcriptome
- Publisher
- TAYLOR &
FRANCIS LTD
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- The time-sequential change in immune-related gene expression of the glioblastoma cell line after irradiation was evaluated to speculate the effect of combined immunotherapy with radiotherapy. The U373 MG glioblastoma cell line was irradiated with 6 Gy single dose. Next-generation sequencing (NGS) transcriptome data was generated before irradiation (control), and at 6, 24, and 48 h post-irradiation. Immune-related pathways were analyzed at each time period. The same analyses were also performed for A549 lung cancer and U87 MG glioblastoma cell lines. Western blotting confirmed the programmed death-ligand 1 (PD-L1) expression levels over time. In the U373 MG cell line, neutrophil-mediated immunity, type I interferon signaling, antigen cross-presentation to T cell, and interferon-gamma signals began to increase significantly at 24 h and were upregulated until 48 h after irradiation. The results were similar to those of the A549 and U87 MG cell lines. Without T cell infiltration, PD-L1 did not increase even with upregulated interferon-gamma signaling in cancer cells. In conclusions, in the glioblastoma cell line, immune-related signals were significantly upregulated at 24 and 48 h after irradiation. Therefore, the time interval between daily radiotherapy might not be enough to expect full immune responses by combined immune checkpoint inhibitors and newly infiltrating immune cells after irradiation.
- DOI
- 10.1080/19768354.2021.1954550
- Appears in Collections:
- 의료원 > 의료원 > Journal papers
- Files in This Item:
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Time sequential change in immune related gene expression after irradiation in glioblastoma next generation sequencing analysis.pdf(1.83 MB)
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