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The core composition of DNA block copolymer micelles dictates DNA hybridization properties, nuclease stabilities, and cellular uptake efficiencies

Title
The core composition of DNA block copolymer micelles dictates DNA hybridization properties, nuclease stabilities, and cellular uptake efficiencies
Authors
Kim, Chan-JinKim, Ga-HyunJeong, Eun HyeLee, HyukjinPark, So-Jung
Ewha Authors
이혁진박소정김찬진정은혜
SCOPUS Author ID
이혁진scopus; 김찬진scopus; 박소정scopus
Issue Date
2021
Journal Title
NANOSCALE
ISSN
2040-3364JCR Link

2040-3372JCR Link
Citation
NANOSCALE vol. 13, no. 32, pp. 13758 - 13763
Publisher
ROYAL SOC CHEMISTRY
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Here, we report how the nature of the hydrophobic core affects the molecular interactions of DNA block copolymer assemblies. Three different amphiphilic DNA block copolymers, DNA-b-polystyrene (DNA-b-PS), DNA-b-poly(2-vinylpyridine) (DNA-b-P2VP), and DNA-b-poly(methyl acrylate) (DNA-b-PMA) were synthesized and assembled into spherical micelles composed of a hydrophobic polymer core and DNA corona. Interestingly, DNA block copolymer micelles having different hydrophobic cores exhibited markedly different molecular and biological interactions. DNA-b-PS exhibited higher melting temperature, sharper melting transition, higher stability to nuclease-catalyzed DNA degradation, and higher cellular uptake efficiency compared to DNA-b-P2VP and DNA-b-PMA. The investigation of the self-assembly behavior revealed a much higher aggregation number and DNA density for DNA-b-PS micelles, which explains the superior properties of DNA-b-PS. These results demonstrate that the type of the hydrophobic core polymer, which has been largely overlooked, has a profound impact on the molecular and biological interactions of the DNA shell.
DOI
10.1039/d1nr00756d
Appears in Collections:
약학대학 > 약학과 > Journal papers
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