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Toll-Like Receptor 7 (TLR7) Mediated Transcriptomic Changes on Human Mast Cells

Title
Toll-Like Receptor 7 (TLR7) Mediated Transcriptomic Changes on Human Mast Cells
Authors
Cho, Kyung-AhChoi, Da-WonPark, MinhwaKim, Yu-HeeWoo, So-Youn
Ewha Authors
우소연조경아김유희
SCOPUS Author ID
우소연scopus; 조경아scopus; 김유희scopus
Issue Date
2021
Journal Title
ANNALS OF DERMATOLOGY
ISSN
1013-9087JCR Link

2005-3894JCR Link
Citation
ANNALS OF DERMATOLOGY vol. 33, no. 5, pp. 402 - 408
Keywords
Mast cellSkin inflammationToll-like receptor 7
Publisher
KOREAN DERMATOLOGICAL ASSOC
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Background: Mast cells are skin immune sentinels located in the upper dermis, where wheal formation and sensory nerve stimulation take place. Skin inflammation is occasionally accompanied by mast cell-driven responses with wheals, angioedema, or both. Immunoglobulin E (IgE) antibodies are regarded as typical stimuli to drive mast cell activation. However, various causative factors, including microbial infections, can drive IgE-independent mast cell response. When infected, the innate immunity orchestrates an immune response by activating receptor signaling via Toll-like receptors (TLRs). Objective: In this study, we determined the effect of TLR7 stimulation on mast cells to investigate the possible mechanism of IgE-independent inflammatory response. Methods: Human mast cell (HMC) line, HMC-1 cells were treated with TLR7 agonist and the morphologic alteration was observed in transmission electron microscopy. Further, TLR7 agonist treated HMC-1 cells were conducted to RNA sequencing to compare transcriptomic features. Results: HMC-1 cells treated with TLR7 agonist reveals increase of intracellular vesicles, lipid droplets, and ribosomes. Also, genes involved in pro-inflammatory responses such as angiogenesis are highly expressed, and Il12rb2 was the most highly up regulated gene. Conclusion: Our data suggest that TLR7 signaling on mast cells might be a potential therapeutic target for mast cell-driven, IgE-independent skin inflammation. (Ann Dermatol 33(5) 402 similar to 408, 2021)
DOI
10.5021/ad.2021.33.5.402
Appears in Collections:
의과대학 > 의학과 > Journal papers
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