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NaHCO3- and NaCl-Type Hot Springs Enhance the Secretion of Inflammatory Cytokine Induced by Polyinosinic-Polycytidylic Acid in HaCaT Cells

Title
NaHCO3- and NaCl-Type Hot Springs Enhance the Secretion of Inflammatory Cytokine Induced by Polyinosinic-Polycytidylic Acid in HaCaT Cells
Authors
Park, Sang HoJung, Bom YeeLee, Soo YoungYu, Dong SooWoo, So-YounYun, Seong-TaekLee, Jong TaeKim, Jin-WouLee, Young Bok
Ewha Authors
우소연
SCOPUS Author ID
우소연scopus
Issue Date
2021
Journal Title
ANNALS OF DERMATOLOGY
ISSN
1013-9087JCR Link

2005-3894JCR Link
Citation
ANNALS OF DERMATOLOGY vol. 33, no. 5, pp. 440 - 447
Keywords
CytokinesHot SpringsInflammationKeratinocytesPoly I-CToll-like receptors
Publisher
KOREAN DERMATOLOGICAL ASSOC
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Background: Hot springs have been traditionally used as an alternative treatment for a wide range of diseases, including rheumatoid arthritis, bronchial asthma, diabetes, hypertension, psoriasis and atopic dermatitis. However, the clinical effects and therapeutic mechanisms associated with hot springs remain poorly defined. Objective: The purpose of this study was to demonstrate the different effects of hot springs on cellular viability and secretion of inflammatory cytokines on keratinocyte in two geographically representative types of hot springs: NaHCO3-type and NaCl-type, which are the most common types in South Korea. Methods: We performed WST-1, BrdU measurements, human inflammatory cytokine arrays and enzyme-linked immunosorbent assay in HaCaT cells stimulated with toll-like receptor 3 by polyinosinic-polycytidylic acid. Results: The interaction effects of cell viability and cell proliferation were not significantly different regardless of polyinosinic-polycytidylic acid stimulation and cultured hot springs type. Cytokine array and enzyme-linked immunosorbent assay analysis showed increased expression of inflammatory cytokines such as interleukin-6 and granulocyte-macrophage colony-stimulating factor by polyinosinic-polycytidylic acid stimulation, with expression levels differing according to hot springs hydrochemical composition. Cytokine reduction was not significant. Conclusion: The effects and mechanisms of hot springs treatment in keratinocytes were partially elucidated.
DOI
10.5021/ad.2021.33.5.440
Appears in Collections:
의과대학 > 의학과 > Journal papers
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