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Long-term effects of pro-opiomelanocortin methylation induced in food-restricted dams on metabolic phenotypes in male rat offspring

Title
Long-term effects of pro-opiomelanocortin methylation induced in food-restricted dams on metabolic phenotypes in male rat offspring
Authors
Lee S.Kwon E.J.You Y.-A.Du J.E.Jo I.Kim Y.J.
Ewha Authors
김영주조인호
SCOPUS Author ID
김영주scopus; 조인호scopus
Issue Date
2020
Journal Title
Obstetrics and Gynecology Science
ISSN
2287-8572JCR Link
Citation
Obstetrics and Gynecology Science vol. 63, no. 3, pp. 239 - 250
Keywords
Appetite regulationDNA methylationMalnutritionMetabolic syndromePro-opiomelanocortin
Publisher
Korean Society of Obstetrics and Gynecology
Indexed
SCOPUS; KCI scopus
Document Type
Article
Abstract
Objective Maternal malnutrition affects the growth and metabolic health of the offspring. Little is known about the longterm effect on metabolic indices of epigenetic changes in the brain caused by maternal diet. Thus, we explored the effect of maternal food restriction during pregnancy on metabolic profiles of the offspring, by evaluating the DNA methylation of hypothalamic appetite regulators at 3 weeks of age. Methods Sprague-Dawley rats were divided into 2 groups: a control group and a group with a 50% food-restricted (FR) diet during pregnancy. Methylation and expression of appetite regulator genes were measured in 3-week-old offspring using pyrosequencing, real-time polymerase chain reaction, and western blotting analyses. We analyzed the relationship between DNA methylation and metabolic profiles by Pearson's correlation analysis. Results The expression of pro-opiomelanocortin (POMC) decreased, whereas DNA methylation significantly increased in male offspring of the FR dams, compared to the male offspring of control dams. Hypermethylation of POMC was positively correlated with the levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol in 3-week-old male offspring. In addition, there were significant positive correlations between hypermethylation of POMC and the levels of triglycerides, HDL-C, and leptin in 6-month-old male offspring. Conclusion Our findings suggest that maternal food restriction during pregnancy influences the expression of hypothalamic appetite regulators via epigenetic changes, leading to the development of metabolic disorders in the offspring. © 2020 Korean Society of Obstetrics and Gynecology.
DOI
10.5468/OGS.2020.63.3.239
Appears in Collections:
의과대학 > 의학과 > Journal papers
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