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Gold nanoparticle-assisted SELEX as a visual monitoring platform for the development of small molecule-binding DNA aptasensors
- Title
- Gold nanoparticle-assisted SELEX as a visual monitoring platform for the development of small molecule-binding DNA aptasensors
- Authors
- Lee, Eun-Song; Kim, Eun-Ji; Park, Tae-Ki; Bae, Da-Woon; Cha, Sun-Shin; Kim, Tae-Wuk; Kim, Young-Pil
- Ewha Authors
- 차선신
- SCOPUS Author ID
- 차선신
- Issue Date
- 2021
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- ISSN
- 0956-5663
1873-4235
- Citation
- BIOSENSORS & BIOELECTRONICS vol. 191
- Keywords
- Aptamers; Gold nanoparticle; SELEX; Biosensor; Colorimetry; Brassinolide; Bisphenol
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- To resolve time-consuming and imperceptible monitoring problems in the traditional systematic evolution of ligands by exponential enrichment (SELEX), we report gold nanoparticle-assisted SELEX (GNP-SELEX) as a visual, proofreading, and self-monitoring platform and its application to small molecule-binding single-stranded DNA (ssDNA) aptasensors. Through the colorimetric changes between rounds, GNP-SELEX enabled the rapid determination of target-specific aptamer library enrichment with neither target modification nor extra monitoring process. We identified ssDNA aptamers with high selectivity and binding affinity by targeting two small molecules (brassinolide; BL and bisphenol A; BPA) as a model. The rational design of selected aptamers by 3D molecular simulation increased their ability to detect BL or BPA in real samples as bioreceptors. These results suggest that GNP-SELEX is useful as a self-monitoring platform to discover ssDNA aptamers as well as to develop aptasensors for diverse targets in a rapid and simple way.
- DOI
- 10.1016/j.bios.2021.113468
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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