Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shunichi Fukuzumi | * |
dc.date.accessioned | 2021-05-28T16:30:39Z | - |
dc.date.available | 2021-05-28T16:30:39Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 0912-0009 | * |
dc.identifier.issn | 1880-5086 | * |
dc.identifier.other | OAK-29150 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/257408 | - |
dc.description.abstract | The effects of reaction environments on the radical-scavenging mechanisms of ascorbic acid (AscH(2)) were investigated using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) as a reactivity model of reactive oxygen species. Water-insoluble DPPH center dot was solubilized by beta-cyclodextrin (beta-CD) in water. The DPPH center dot-scavenging rate of AscH(2) in methanol (MeOH) was much slower than that in phosphate buffer (0.05 M, pH 7.0). An organic soluble 5,6-isopropylidene-Lascorbic acid (iAscH(2)) scavenged DPPH center dot much slower in acetonitrile (MeCN) than in MeOH. In MeOH, Mg(ClO4)(2) significantly decelerated the DPPH center dot-scavenging reaction by AscH(2) and iAscH(2), while no effect of Mg(ClO4)(2) was observed in MeCN. On the other hand, Mg(ClO4)(2) significantly accelerated the reaction between AscH(2) and beta-CD-solubilized DPPH center dot (DPPH center dot/beta-CD) in phosphate buffer (0.05 M, pH 6.5), although the addition of 0.05 M Mg(ClO4)(2) to the AscH(2)-DPPH center dot/beta-CD system in phosphate buffer (0.05 M, pH 7.0) resulted in the change in pH of the phosphate buffer to be 6.5. Thus, the DPPH center dot-scavenging reaction by iAscH(2) in MeCN may proceed via a one-step hydrogen-atom transfer, while an electrontransfer pathway is involved in the reaction between AscH(2) and DPPH center dot/beta-CD in phosphate buffer solution. These results demonstrate that the DPPH center dot-scavenging mechanism of AscH(2) are affected by the reaction environments. | * |
dc.language | English | * |
dc.publisher | JOURNAL CLINICAL BIOCHEMISTRY & | * |
dc.publisher | NUTRITION | * |
dc.subject | antioxidant | * |
dc.subject | ascorbic acid | * |
dc.subject | radical | * |
dc.subject | reaction mechanism | * |
dc.subject | hydrogen transfer | * |
dc.title | Effects of reaction environments on radical-scavenging mechanisms of ascorbic acid | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 68 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 116 | * |
dc.relation.lastpage | 122 | * |
dc.relation.journaltitle | JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION | * |
dc.identifier.doi | 10.3164/jcbn.20-147 | * |
dc.identifier.wosid | WOS:000687886700002 | * |
dc.identifier.scopusid | 2-s2.0-85102249525 | * |
dc.author.google | Nakanishi, Ikuo | * |
dc.author.google | Shoji, Yoshimi | * |
dc.author.google | Ohkubo, Kei | * |
dc.author.google | Fukuhara, Kiyoshi | * |
dc.author.google | Ozawa, Toshihiko | * |
dc.author.google | Matsumoto, Ken-ichiro | * |
dc.author.google | Fukuzumi, Shunichi | * |
dc.contributor.scopusid | Shunichi Fukuzumi(35430038100;58409757400) | * |
dc.date.modifydate | 20240401081001 | * |