Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이화정 | * |
dc.date.accessioned | 2021-02-25T16:31:53Z | - |
dc.date.available | 2021-02-25T16:31:53Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1942-7603 | * |
dc.identifier.other | OAK-28858 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/257179 | - |
dc.description.abstract | Human insulin-like growth factor 1 (IGF-I) is the primary mediator of the effects of the growth hormone (GH). Therefore, it has been used as a biomarker to detect the abuse of GH in sports. The measurement of IGF-I relies on mass-based and immunological approaches to analysis. Among the mass-based analysis methods, liquid chromatography–mass spectrometry (LC–MS) has a number of functional advantages. LC–MS measurements based on the quantification of IGF-I, according to trypsin digestion, are used in the most common method of analyzing doping. However, this method is time-consuming and subject to experimental variability. In this study, we optimized a rapid method for detecting IGF-I without the trypsin digestion step. This method of analysis uses an ultra-centrifugal filter and an LC-HRMS through narrow-range mass scan method. To verify the validity of this method, eight categories of validation testing were applied with the following results: linearity, R2 > 0.99; limit of detection, 15 ng/ml; limit of quantification, 20 ng/ml; accuracy, >99%; recovery rate, >95%; carryover, <0.03; and inter- and intra-day precision values, %CV < 2% and %CV < 6%, respectively. Furthermore, we discussed the correlation of the quantified concentration from two other methods, immunoradiometric assay (IRMA) and parallel reaction monitoring method, using 209 serum samples. In conclusion, although both mass spectrometry-based methods worked equally well in terms of analytical performance and correlation with IRMA results, narrow-range mass scan method had several advantages, such as time and cost savings and reliable reproducibility, over the existing methods. © 2020 John Wiley & Sons, Ltd. | * |
dc.language | English | * |
dc.publisher | John Wiley and Sons Ltd | * |
dc.subject | doping control analysis | * |
dc.subject | hGH biomarker | * |
dc.subject | IGF-I | * |
dc.subject | method validation | * |
dc.subject | narrow-range mass scan | * |
dc.title | Optimization, validation, and comparison of a rapid method for the quantification of insulin-like growth factor 1 in serum using liquid chromatography–high-resolution mass spectrometry | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 13 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 451 | * |
dc.relation.lastpage | 459 | * |
dc.relation.journaltitle | Drug Testing and Analysis | * |
dc.identifier.doi | 10.1002/dta.2944 | * |
dc.identifier.scopusid | 2-s2.0-85093849811 | * |
dc.author.google | Seo Y. | * |
dc.author.google | Park J. | * |
dc.author.google | Kim M. | * |
dc.author.google | Sung C. | * |
dc.author.google | Kwon O.-S. | * |
dc.author.google | Lee H.J. | * |
dc.author.google | Min H. | * |
dc.contributor.scopusid | 이화정(57102029300) | * |
dc.date.modifydate | 20240118154655 | * |