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Optimization, validation, and comparison of a rapid method for the quantification of insulin-like growth factor 1 in serum using liquid chromatography–high-resolution mass spectrometry
- Optimization, validation, and comparison of a rapid method for the quantification of insulin-like growth factor 1 in serum using liquid chromatography–high-resolution mass spectrometry
- Seo Y.; Park J.; Kim M.; Sung C.; Kwon O.-S.; Lee H.J.; Min H.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Drug Testing and Analysis
- Drug Testing and Analysis vol. 13, no. 2, pp. 451 - 459
- doping control analysis; hGH biomarker; IGF-I; method validation; narrow-range mass scan
- John Wiley and Sons Ltd
- SCIE; SCOPUS
- Document Type
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- Human insulin-like growth factor 1 (IGF-I) is the primary mediator of the effects of the growth hormone (GH). Therefore, it has been used as a biomarker to detect the abuse of GH in sports. The measurement of IGF-I relies on mass-based and immunological approaches to analysis. Among the mass-based analysis methods, liquid chromatography–mass spectrometry (LC–MS) has a number of functional advantages. LC–MS measurements based on the quantification of IGF-I, according to trypsin digestion, are used in the most common method of analyzing doping. However, this method is time-consuming and subject to experimental variability. In this study, we optimized a rapid method for detecting IGF-I without the trypsin digestion step. This method of analysis uses an ultra-centrifugal filter and an LC-HRMS through narrow-range mass scan method. To verify the validity of this method, eight categories of validation testing were applied with the following results: linearity, R2 > 0.99; limit of detection, 15 ng/ml; limit of quantification, 20 ng/ml; accuracy, >99%; recovery rate, >95%; carryover, <0.03; and inter- and intra-day precision values, %CV < 2% and %CV < 6%, respectively. Furthermore, we discussed the correlation of the quantified concentration from two other methods, immunoradiometric assay (IRMA) and parallel reaction monitoring method, using 209 serum samples. In conclusion, although both mass spectrometry-based methods worked equally well in terms of analytical performance and correlation with IRMA results, narrow-range mass scan method had several advantages, such as time and cost savings and reliable reproducibility, over the existing methods. © 2020 John Wiley & Sons, Ltd.
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