View : 121 Download: 0

Ex vivo live full-thickness porcine skin model as a versatile in vitro testing method for skin barrier research

Title
Ex vivo live full-thickness porcine skin model as a versatile in vitro testing method for skin barrier research
Authors
Hwang J.-H.Jeong H.Lee N.Hur S.Han J.J.Jang H.W.Choi W.K.Nam K.T.Lim K.-M.
Ewha Authors
임경민
SCOPUS Author ID
임경민scopus
Issue Date
2021
Journal Title
International Journal of Molecular Sciences
ISSN
1661-6596JCR Link
Citation
International Journal of Molecular Sciences vol. 22, no. 2, pp. 1 - 16
Keywords
Ex vivo skin modelHydroxyacidsSkin barrierSkin permeabilityStratum corneum
Publisher
MDPI AG
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Since the European Union (EU) announced their animal testing ban in 2013, all animal experiments related to cosmetics have been prohibited, creating a demand for alternatives to animal experiments for skin studies. Here, we investigated whether an ex vivo live porcine skin model can be employed to study the safety and skin barrier-improving effects of hydroxyacids widely used in cosmetics for keratolytic peels. Glycolic acid (1–10%), salicylic acid (0.2–2%), and lactobionic acid (1.2–12%) were used as representative substances for α-hydroxyacid (AHA), β-hydroxyacid (BHA), and polyhydroxyacid (PHA), respectively. When hydroxyacids were applied at high concentrations on the porcine skin every other day for 6 days, tissue viability was reduced to 50–80%, suggesting that the toxicity of cosmetic ingredients can be evaluated with this model. Based on tissue viability, the treatment scheme was changed to a single exposure for 20 min. The protective effects of a single exposure of hydroxyacids on skin barrier function were evaluated by examining rhodamine permeability and epidermal structural components of barrier function using immunohistochemistry (IHC) and immunofluorescence (IF) staining. Lactobionic acid (PHAs) improved skin barrier function most compared to other AHAs and BHAs. Most importantly, trans-epidermal water loss (TEWL), an important functional marker of skin barrier function, could be measured with this model, which confirmed the significant skin barrier-protective effects of PHAs. Collectively, we demonstrated that the ex vivo live full-thickness porcine skin model can be an excellent alternative to animal experiments for skin studies on the safety and efficacy of cosmetic ingredients. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Show the fulltext
DOI
10.3390/ijms22020657
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE