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Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization

Title
Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization
Authors
Saratale, Rijuta GaneshCho, Si KyungSaratale, Ganesh DattatrayaGhodake, Gajanan S.Bharagava, Ram NareshKim, Dong SuNair, SupriyaShin, Han Seung
Ewha Authors
김동수
SCOPUS Author ID
김동수scopus
Issue Date
2021
Journal Title
BIORESOURCE TECHNOLOGY
ISSN
0960-8524JCR Link

1873-2976JCR Link
Citation
BIORESOURCE TECHNOLOGY vol. 324
Keywords
Lysinibacillus sp. RGSSugarcane bagassePeracetic acid pretreatmentPoly(3-hydroxybutyrate)Saccharification yieldCorn steep liquor
Publisher
ELSEVIER SCI LTD
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 degrees C, pH 7; inoculums concentration (2.5% v/ v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.
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DOI
10.1016/j.biortech.2021.124673
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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