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Double cocktail immunostains with high molecular weight cytokeratin and GATA-3: Useful stain to discriminate in situ involvement of prostatic ducts or acini from stromal invasion by urothelial carcinoma in the prostate

Title
Double cocktail immunostains with high molecular weight cytokeratin and GATA-3: Useful stain to discriminate in situ involvement of prostatic ducts or acini from stromal invasion by urothelial carcinoma in the prostate
Authors
Lee J.Yoo Y.Park S.Cho M.-S.Sung S.H.Ro J.Y.
Ewha Authors
성순희조민선박상희
SCOPUS Author ID
성순희scopus; 조민선scopus; 박상희scopus
Issue Date
2020
Journal Title
Journal of Pathology and Translational Medicine
ISSN
2383-7837JCR Link
Citation
Journal of Pathology and Translational Medicine vol. 54, no. 2, pp. 146 - 153
Keywords
GATA-3High molecular weight cytokeratinProstatic stromal invasionUrothelial carcinoma
Publisher
Seoul National University
Indexed
SCOPUS; KCI scopus
Document Type
Article
Abstract
Background: Distinguishing prostatic stromal invasion (PSI) by urothelial carcinoma (UC) from in situ UC involving prostatic ducts or acini with no stromal invasion (in situ involvement) may be challenging on hematoxylin and eosin stained sections. However, the distinction between them is important because cases with PSI show worse prognosis. This study was performed to assess the utility of double cocktail immunostains with high molecular weight cytokeratin (HMWCK) and GATA-3 to discriminate PSI by UC from in situ UC involvement of prostatic ducts or acini in the prostate. Methods: Among 117 radical cystoprostatectomy specimens for bladder UCs, 25 cases showed secondary involvement of bladder UC in prostatic ducts/acini only or associated stromal invasion and of these 25 cases, seven cases revealed equivocal PSI. In these seven cases with equivocal PSI, HMWCK, and GATA-3 double immunohistochemical stains were performed to identify whether this cocktail stain is useful to identify the stromal invasion. Results: In all cases, basal cells of prostate glands showed strong cytoplasmic staining for HMWCK and UC cells showed strong nuclear staining for GATA-3. In cases with stromal invasion of UC, GATA-3-positive tumor cells in the prostatic stroma without surrounding HMWCK-positive basal cells were highlighted and easily recognized. Among seven equivocal cases, two cases showed PSI and five in situ UC in the prostate. In two cases, the original diagnoses were revised. Conclusions: Our study suggested that HMWCK and GATA-3 double stains could be utilized as an adjunct method in the distinction between PSI by UC from in situ UC involving prostatic ducts or acini. © 2020 The Korean Society of Pathologists/The Korean Society for Cytopathology
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DOI
10.4132/jptm.2019.11.12
Appears in Collections:
의과대학 > 의학과 > Journal papers
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