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LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1
- Title
- LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1
- Authors
- Yoo J.-Y.; Cha D.R.; Kim B.; An E.J.; Lee S.R.; Cha J.J.; Kang Y.S.; Ghee J.Y.; Han J.Y.; Bae Y.S.
- Ewha Authors
- 배윤수
- SCOPUS Author ID
- 배윤수
- Issue Date
- 2020
- Journal Title
- Cell Reports
- ISSN
- 2211-1247
- Citation
- Cell Reports vol. 33, no. 3
- Keywords
- AKI; cytokine; H2O2, LPS; inflammation; Nox4; sepsis; SH3YL1; TLR4; tubular damage
- Publisher
- Elsevier B.V.
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Cytosolic proteins are required for regulation of NADPH (nicotinamide adenine dinucleotide phosphate) oxidase (Nox) isozymes. Here we show that Src homology 3 (SH3) domain-containing YSC84-like 1 (SH3YL1), as a Nox4 cytosolic regulator, mediates lipopolysaccharide (LPS)-induced H2O2 generation, leading to acute kidney injury. The SH3YL1, Ysc84p/Lsb4p, Lsb3p, and plant FYVE proteins (SYLF) region and SH3 domain of SH3YL1 contribute to formation of a complex with Nox4-p22phox. Interaction of p22phox with SH3YL1 is triggered by LPS, and the complex induces H2O2 generation and pro-inflammatory cytokine expression in mouse tubular epithelial cells. After LPS injection, SH3YL1 knockout mice show lower levels of acute kidney injury biomarkers, decreased secretion of pro-inflammatory cytokines, decreased infiltration of macrophages, and reduced tubular damage compared with wild-type (WT) mice. The results strongly suggest that SH3YL1 is involved in renal failure in LPS-induced acute kidney injury (AKI) mice. We demonstrate that formation of a ternary complex of p22phox-SH3YL1-Nox4, leading to H2O2 generation, induces severe renal failure in the LPS-induced AKI model. Yoo et al. demonstrate that SH3YL1 serves as a cytosolic regulator of Nox4 to mediate LPS-dependent H2O2 generation. The Nox4-SH3YL1 axis stimulates expression of pro-inflammatory cytokines and then triggers apoptosis of tubular cells, leading to AKI. SH3YL1 plays an important role in diseases associated with H2O2 produced by Nox4. © 2020 The Authors
- DOI
- 10.1016/j.celrep.2020.108245
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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