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Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells

Title
Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells
Authors
Park, Hyo-JungBang, Eun-KyoungHong, Jung JooLee, Sang-MyeongKo, Hae LiKwak, Hye WonPark, HyelimKang, Kyung WonKim, Rhoon-HoRyu, Seung RokKim, GreenOh, HanseulKim, Hye-JungLee, KyuriKim, MinjeongKim, Soo YoungKim, Jae-OukEl-Baz, KarimLee, HyukjinSong, MankiJeong, Dae GwinKeum, GyochangNam, Jae-Hwan
Ewha Authors
이혁진이규리김민정
SCOPUS Author ID
이혁진scopus; 김민정scopus
Issue Date
2020
Journal Title
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN
1433-7851JCR Link

1521-3773JCR Link
Citation
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION vol. 59, no. 28, pp. 11540 - 11549
Keywords
amphiphilesantigensnanoparticlesRNA structuresviruses
Publisher
WILEY-V C H VERLAG GMBH
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
As agonists of TLR7/8, single-stranded RNAs (ssRNAs) are safe and promising adjuvants that do not cause off-target effects or innate immune overactivation. However, low stability prevents them from mounting sufficient immune responses. This study evaluates the adjuvant effects of ssRNA derived from the cricket paralysis virus intergenic region internal ribosome entry site, formulated as nanoparticles with a coordinative amphiphile, containing a zinc/dipicolylamine complex moiety as a coordinative phosphate binder, as a stabilizer for RNA-based adjuvants. The nanoformulated ssRNA adjuvant was resistant to enzymatic degradation in vitro and in vivo, and that with a coordinative amphiphile bearing an oleyl group (CA-O) was approximately 100 nm, promoted effective recognition, and improved activation of antigen-presenting cells, leading to better induction of neutralizing antibodies following single immunization. Hence,CA-Omay increase the efficacy of ssRNA-based adjuvants, proving useful to meet the urgent need for vaccines during pathogen outbreaks.
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DOI
10.1002/ange.202002979
Appears in Collections:
약학대학 > 약학과 > Journal papers
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