Raineyella fluvialis sp. nov., an actinobacterium isolated from freshwater sediment

Abstract

A novel, facultatively anaerobic actinobacterium, designated strain CBA3103(T), was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103(T) is most closely related to Raineyella antarctica LZ-22(T) (98.47% 16S rRNA gene sequence similarity). The genome of strain CBA3103(T) was 3 649 865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103(T) and R. antarctica LZ-22T was 79.22 %. Cells of strain CBA3103(T) were Gram-positive, rod-shaped, 0.6-0.9 mu m wide and 1.4-2.4 mu m long. Growth occurred at 15-40 degrees C (optimum, 35 degrees C), at pH 6.0-7.0 (optimum, pH 7.0) and with 0-2 % NaCl (w/v) (optimum, 0-1 %, w/v). The major cellular fatty acids in strain CBA3103(T) were anteiso-C-15:0, anteiso-C-15:1 A and iso-C-14:0. The major respiratory quinone was menaquinone-9(H-4). The polar lipids of strain CBA3103(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103(T) represents a novel species of the genus Raineyella, for which the name Raineyella fluvialis sp. nov. (type strain CBA3103(T)=KACC 21446(T)=DSM 110288(T)) is proposed.