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dc.contributor.author박시재-
dc.date.accessioned2020-03-23T16:30:20Z-
dc.date.available2020-03-23T16:30:20Z-
dc.date.issued2020-
dc.identifier.issn0141-8130-
dc.identifier.issn1879-0003-
dc.identifier.otherOAK-26579-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/253623-
dc.description.abstractSucrose utilization has been established in Escherichia coil strains by expression of Mannheimia succiniciproducens beta-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coil strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) IP( 3HB)1 and poly(3-hydroxybutyrate-co-lactate) (P(3HB-co-LA)] from sucrose. When recombinant E. coil strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coil strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P (3H13-co-1A) from sucrose. Among the examined E. coli strains, recombinant E. coil XL1-Blue produced the highest contents of P(3HB) (53.60 +/- 255 wt%) and P(3HB-co-LA) (29.44 +/- 039 wt%). In the batch fermentations, recombinant E. coil XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt%P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a costefficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source. (C) 2020 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER-
dc.subjectEscherichia coli-
dc.subjectSucrose-
dc.subjectPolyhydroxyalkanoates-
dc.titleBiosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains-
dc.typeArticle-
dc.relation.volume149-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage593-
dc.relation.lastpage599-
dc.relation.journaltitleINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES-
dc.identifier.doi10.1016/j.ijbiomac.2020.01.254-
dc.identifier.wosidWOS:000525795400059-
dc.identifier.scopusid2-s2.0-85078829301-
dc.author.googleSohn, Yu Jung-
dc.author.googleKim, Hee Taek-
dc.author.googleBaritugo, Kei-Anne-
dc.author.googleSong, Hye Min-
dc.author.googleRyu, Mi Hee-
dc.author.googleKang, Kyoung Hee-
dc.author.googleJo, Seo Young-
dc.author.googleKim, Hoyong-
dc.author.googleKim, You Jin-
dc.author.googleChoi, Jong-il-
dc.author.googlePark, Su Kyeong-
dc.author.googleJoo, Jeong Chan-
dc.author.googlePark, Si Jae-
dc.contributor.scopusid박시재(57193344464)-
dc.date.modifydate20200608111936-
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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