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Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains

Title
Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains
Authors
Sohn, Yu JungKim, Hee TaekBaritugo, Kei-AnneSong, Hye MinRyu, Mi HeeKang, Kyoung HeeJo, Seo YoungKim, HoyongKim, You JinChoi, Jong-ilPark, Su KyeongJoo, Jeong ChanPark, Si Jae
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2020
Journal Title
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN
0141-8130JCR Link

1879-0003JCR Link
Citation
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES vol. 149, pp. 593 - 599
Keywords
Escherichia coliSucrosePolyhydroxyalkanoates
Publisher
ELSEVIER
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Sucrose utilization has been established in Escherichia coil strains by expression of Mannheimia succiniciproducens beta-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coil strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) IP( 3HB)1 and poly(3-hydroxybutyrate-co-lactate) (P(3HB-co-LA)] from sucrose. When recombinant E. coil strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coil strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P (3H13-co-1A) from sucrose. Among the examined E. coli strains, recombinant E. coil XL1-Blue produced the highest contents of P(3HB) (53.60 +/- 255 wt%) and P(3HB-co-LA) (29.44 +/- 039 wt%). In the batch fermentations, recombinant E. coil XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt%P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a costefficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source. (C) 2020 Elsevier B.V. All rights reserved.
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DOI
10.1016/j.ijbiomac.2020.01.254
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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