Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 강덕희 | - |
dc.contributor.author | 김달아 | - |
dc.contributor.author | 류정화 | - |
dc.date.accessioned | 2020-02-10T16:30:09Z | - |
dc.date.available | 2020-02-10T16:30:09Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.issn | 1530-6860 | - |
dc.identifier.other | OAK-26317 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253389 | - |
dc.description.abstract | Recent data suggested a causative role of uric acid (UA) in the development of renal disease, in which endothelial dysfunction is regarded as the key mechanism. Endothelial-to-mesenchymal transition (EndoMT) and shedding of the glycocalyx are early changes of endothelial dysfunction. We investigated whether UA induced EndoMT in HUVECs and an animal model of hyperuricemia fed with 2% oxonic acid for 4 wk. UA induced EndoMT in HUVECs with a generation of reactive oxygen species via the activation of membranous NADPH oxidase (from 15 min) and mitochondria (from 6 h) along with glycocalyx shedding (from 6 h), which were blocked by probenecid. GM6001, an inhibitor of matrix metalloproteinase, alleviated UA-induced glycocalyx shedding and EndoMT. Antioxidants including N-acetyl cysteine, apocynin, and mitotempo ameliorated EndoMT; however, they did not change glycocalyx shedding in HUVECs. In the kidney of hyperuricemic rats, endothelial staining in peritubular capillaries (PTCs) was substantially decreased with a de novo expression of alpha-smooth muscle actin in PTCs. Plasma level of syndecan-1 was increased in hyperuricemic rats, which was ameliorated by allopurinol. UA caused a phenotypic transition of endothelial cells via induction of oxidative stress with glycocalyx shedding, which could be one of the mechanisms of UA-induced endothelial dysfunction and kidney disease. | - |
dc.language | English | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.subject | EndoMT | - |
dc.subject | reactive oxygen species | - |
dc.subject | hyperuricemia | - |
dc.subject | syndecan-1 | - |
dc.title | Uric acid induced the phenotype transition of vascular endothelial cells via induction of oxidative stress and glycocalyx shedding | - |
dc.type | Article | - |
dc.relation.issue | 12 | - |
dc.relation.volume | 33 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 13334 | - |
dc.relation.lastpage | 13345 | - |
dc.relation.journaltitle | FASEB JOURNAL | - |
dc.identifier.doi | 10.1096/fj.201901148R | - |
dc.identifier.wosid | WOS:000507466100024 | - |
dc.identifier.scopusid | 2-s2.0-85075962441 | - |
dc.author.google | Ko, Jiyeon | - |
dc.author.google | Kang, Hyun-Jung | - |
dc.author.google | Kim, Dal-Ah | - |
dc.author.google | Kim, Mi-Jin | - |
dc.author.google | Ryu, Eun-Sun | - |
dc.author.google | Lee, Shina | - |
dc.author.google | Ryu, Jung-Hwa | - |
dc.author.google | Roncal, Carlos | - |
dc.author.google | Johnson, Richard J. | - |
dc.author.google | Kang, Duk-Hee | - |
dc.contributor.scopusid | 강덕희(17233695600) | - |
dc.contributor.scopusid | 김달아(57193326495) | - |
dc.date.modifydate | 20230401081003 | - |