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Internalization and Transportation of Endothelial Cell Surface K(Ca)2.3 and K(Ca)3.1 in Normal Pregnancy and Preeclampsia
- Title
- Internalization and Transportation of Endothelial Cell Surface K(Ca)2.3 and K(Ca)3.1 in Normal Pregnancy and Preeclampsia
- Authors
- Choi, Shinkyu; Kim, Ji Aee; Oh, Seikwan; Park, Mi Hye; Cho, Geum Joon; Suh, Suk Hyo
- Ewha Authors
- 서석효; 오세관; 박미혜; 최신규
- SCOPUS Author ID
- 서석효; 오세관; 박미혜; 최신규
- Issue Date
- 2019
- Journal Title
- OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
- ISSN
- 1942-0900
1942-0994
- Citation
- OXIDATIVE MEDICINE AND CELLULAR LONGEVITY vol. 2019
- Publisher
- HINDAWI LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Altered redox state modulates the expression levels of endothelial K(Ca)2.3 and K(Ca)3.1 (K(Ca)s) in normal pregnancy (NP) and preeclampsia (PE), thereby regulating vascular contractility. The mechanisms underlying K(Ca)s endocytosis and transportation remain unknown. We investigated the regulation of K(Ca)s expression in plasma membrane (PM) during NP and PE. Cultured human uterine artery endothelial cells were incubated in serum from normal nonpregnant women and women with NP or PE, or in oxidized LDL-, or lysophosphatidylcholine- (LPC-) containing a medium for 24 hours. NP serum elevated PM levels of K(Ca)s and reduced caveolin-1 and clathrin levels. PE serum, oxidized LDL, or LPC reduced PM levels of K(Ca)s and elevated caveolin-1, clathrin, Rab5c, and early endosome antigen-1 (EEA1) levels. Reduced K(Ca)s levels by PE serum or LPC were reversed by inhibition of caveolin-1, clathrin, or EEA1. Catalase and glutathione peroxidase 1 (GPX1) knockdown elevated PM-localized K(Ca)s levels and reduced caveolin-1 and clathrin levels. Elevated K(Ca)2.3 levels upon catalase and GPX1 knockdown were reversed by PEG-catalase treatment. An H2O2 donor reduced clathrin and Rab5c. In contrast, elevated clathrin, caveolin-1, or colocalization of caveolin-1 with K(Ca)3.1 by PE serum or LPC was reversed by NADPH oxidase inhibitors or antioxidants. A superoxide donor xanthine+xanthine oxidase elevated caveolin-1 or Rab5c levels. We concluded that K(Ca)s are endocytosed in a caveola- or a clathrin-dependent manner and transported in a Rab5c- and EEA1-dependent manner during pregnancy. The endocytosis and transportation processes may slow down via H2O2-mediated pathways in NP and may be accelerated via superoxide-mediated pathways in PE.
- DOI
- 10.1155/2019/5820839
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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Internalization and Transportation of Endothelial Cell Surface.pdf(4.19 MB)
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