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Over-expression of p190RhoGEF enhances B-cell activation and germinal center formation in T-cell-dependent humoral immune responses
- Title
- Over-expression of p190RhoGEF enhances B-cell activation and germinal center formation in T-cell-dependent humoral immune responses
- Authors
- Jeong, Ji Hye; Ha, Yun Jung; Choi, So-Yeon; Kim, Jee-Hae; Yun, Yungdae; Lee, Jong Ran
- Ewha Authors
- 이종란; 윤영대
- SCOPUS Author ID
- 이종란; 윤영대
- Issue Date
- 2019
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- ISSN
- 0818-9641
1440-1711
- Citation
- IMMUNOLOGY AND CELL BIOLOGY vol. 97, no. 10, pp. 877 - 887
- Keywords
- B cells; CD40 signaling; humoral immune responses; p190RhoGEF; plasma cells
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Previously, we reported induced expression of the p190 Rho guanine nucleotide exchange factor (p190RhoGEF, ARHGEF28) following CD40 stimulation of B cells isolated from mouse spleen. We also reported that p190RhoGEF and a downstream effector molecule RhoA are required for B-cell differentiation, especially for the induction of the plasma cell (PC) differentiation. This study investigates the role of p190RhoGEF in B-cell biology in vivo, using p190RhoGEF transgenic (TG) mice that overexpress a wild-type full gene in B cells. Immunization of these mice with T-cell-dependent antigen showed that populations of germinal center B cells and PCs were significantly increased in TG mice. Furthermore, similar results were shown in recombination activating 1 (Rag1) knockout mice that were reconstituted with B cells isolated from TG mice in combination with T cells isolated from littermate control mice. Analyses of isotype class switching and transcription factors involved in a germinal center reaction and PC differentiation also supported the findings from the cellular responses. These results suggest that p190RhoGEF may play a role in the stage of PC differentiation during T-cell-dependent humoral immune responses.
- DOI
- 10.1111/imcb.12286
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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