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dc.contributor.author심현보*
dc.date.accessioned2019-12-03T16:30:32Z-
dc.date.available2019-12-03T16:30:32Z-
dc.date.issued2019*
dc.identifier.issn2218-273X*
dc.identifier.otherOAK-26144*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/252328-
dc.description.abstractColorectal cancer (CRC) is one of the leading causes of cancer death worldwide. Cetuximab, a human/mouse chimeric monoclonal antibody, is effective in a limited number of CRC patients because of cetuximab resistance. This study aimed to identify novel therapeutic targets in cetuximab-resistant CRC in order to improve clinical outcomes. Through phage display technology, we isolated a fully human antibody strongly binding to the cetuximab-resistant HCT116 cell surface and identified the target antigen as glucose-regulated protein 94 (GRP94) using proteomic analysis. Short interfering RNA-mediated GRP94 knockdown showed that GRP94 plays a key role in HCT116 cell growth. In vitro functional studies revealed that the GRP94-blocking antibody we developed strongly inhibits the growth of various cetuximab-resistant CRC cell lines. We also demonstrated that GRP94 immunoglobulin G monotherapy significantly reduces HCT116 cell growth more potently compared to cetuximab, without severe toxicity in vivo. Therefore, cell surface GRP94 might be a potential novel therapeutic target in cetuximab-resistant CRC, and antibody-based targeting of GRP94 might be an effective strategy to suppress GRP94-expressing cetuximab-resistant CRC.*
dc.languageEnglish*
dc.publisherMDPI*
dc.subjectcetuximab resistance*
dc.subjectGRP94*
dc.subjecthuman antibody*
dc.subjectcolorectal cancer*
dc.titleAntibody-Based Targeting of Cell Surface GRP94 Specifically Inhibits Cetuximab-Resistant Colorectal Cancer Growth*
dc.typeArticle*
dc.relation.issue11*
dc.relation.volume9*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.journaltitleBIOMOLECULES*
dc.identifier.doi10.3390/biom9110681*
dc.identifier.wosidWOS:000502267900042*
dc.identifier.scopusid2-s2.0-85074533329*
dc.author.googleJeoung, Mee Hyun*
dc.author.googleKim, Taek-Keun*
dc.author.googleKim, Ji Woong*
dc.author.googleCho, Yea Bin*
dc.author.googleNa, Hee Jun*
dc.author.googleYoo, Byong Chul*
dc.author.googleShim, Hyunbo*
dc.author.googleSong, Dong-Keun*
dc.author.googleHeo, Kyun*
dc.author.googleLee, Sukmook*
dc.contributor.scopusid심현보(26635827900)*
dc.date.modifydate20240123110611*


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