Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김양하 | * |
dc.contributor.author | 장유진 | * |
dc.date.accessioned | 2019-12-03T16:30:28Z | - |
dc.date.available | 2019-12-03T16:30:28Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 2072-6643 | * |
dc.identifier.other | OAK-26167 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252310 | - |
dc.description.abstract | Excessive fat accumulation has been considered as a major contributing factor for muscle mitochondrial dysfunction and its associated metabolic complications. The purpose of present study is to investigate a role of vitamin D in muscle fat accumulation and mitochondrial changes. In differentiated C2C12 muscle cells, palmitic acid (PA) was pretreated, followed by incubation with 1,25-dihyroxyvitamin D (1,25(OH)2D) for 24 h. PA led to a significant increment of triglyceride (TG) levels with increased lipid peroxidation and cellular damage, which were reversed by 1,25(OH)2D. The supplementation of 1,25(OH)2D significantly enhanced PA-decreased mtDNA levels as well as mRNA levels involved in mitochondrial biogenesis such as nuclear respiratory factor 1 (NRF1), peroxisome proliferative activated receptor gamma coactivator-1 alpha (PGC-1 alpha), and mitochondrial transcription factor A (Tfam) in C2C12 myotubes. Additionally, 1,25(OH)2D significantly increased ATP levels and gene expression related to mitochondrial function such as carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR alpha), very long-chain acyl-CoA dehydrogenase (VLCAD), long-chain acyl-CoA dehydrogenase (LCAD), medium-chain acyl-CoA dehydrogenase (MCAD), uncoupling protein 2 (UCP2), and UCP3 and the vitamin D pathway including 25-dihydroxyvitamin D3 24-hydroxylase (CYP24) and 25-hydroxyvitamin D3 1-alpha-hydroxylase (CYP27) in PA-treated C2C12 myotubes. In addition to significant increment of sirtuin 1 (SIRT1) mRNA expression, increased activation of adenosine monophosphate-activated protein kinase (AMPK) and SIRT1 was found in 1,25(OH)2D-treated C2C12 muscle cells. Thus, we suggest that the observed protective effect of vitamin D on muscle fat accumulation and mitochondrial dysfunction in a positive manner via modulating AMPK/SIRT1 activation. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | adenosine monophosphate-activated protein kinase (AMPK) | * |
dc.subject | muscle | * |
dc.subject | obesity | * |
dc.subject | sirtulin 1 (SIRT1) | * |
dc.subject | vitamin D | * |
dc.title | Vitamin D Ameliorates Fat Accumulation with AMPK/SIRT1 Activity in C2C12 Skeletal Muscle Cells | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 11 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | NUTRIENTS | * |
dc.identifier.doi | 10.3390/nu11112806 | * |
dc.identifier.wosid | WOS:000502274600257 | * |
dc.identifier.scopusid | 2-s2.0-85075291348 | * |
dc.author.google | Chang, Eugene | * |
dc.author.google | Kim, Yangha | * |
dc.contributor.scopusid | 김양하(8524160300) | * |
dc.contributor.scopusid | 장유진(30367468300) | * |
dc.date.modifydate | 20240123090847 | * |