Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김승정 | * |
dc.contributor.author | 류정화 | * |
dc.date.accessioned | 2019-12-03T16:30:13Z | - |
dc.date.available | 2019-12-03T16:30:13Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 1018-1172 | * |
dc.identifier.issn | 1423-0135 | * |
dc.identifier.other | OAK-26086 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252258 | - |
dc.description.abstract | Vascular access stenosis predominantly occurs as a result of neointimal hyperplasia (NH) formation at the anastomosis. Moreover, in the presence of NH, transforming growth factor-beta (TGF-beta) promotes vascular smooth muscle cell (VSMC) proliferation. Extracellular vesicles (EVs) released by endothelial cells are closely associated with vascular dysfunction. Here, we investigated the effects of EVs on TGF-beta signaling and VSMC proliferation. Specifically, EVs were collected from the culture medium of indoxyl sulfate (IS)-treated human umbilical vein endothelial cells and used (2 x 10(6)) to stimulate human aortic smooth muscle cells (SMCs) (1 x 10(6)). Western blotting was performed to assess the levels of Akt, ERK1/2, p38 MAPK, and Smad3. BrdU proliferation assays, quantitative PCR, and ELISA assays were performed to evaluate SMC proliferation and TGF-beta production. The IS-induced EVs stimulated the proliferation of aortic SMCs in a concentration-dependent manner. The EVs both contained TGF-beta and promoted TGF-beta production by SMCs by phosphorylating Akt, ERK1/2, p38 MAPK, and Smad3, which was significantly inhibited by an anti-TGF-beta antibody. SMC proliferation was suppressed by both an anti-TGF-beta antibody and inhibitors of the downstream factors. These results suggest that EVs are involved in the pathogenesis of vascular access stenosis by modulating TGF-beta signaling in VSMCs under uremic conditions. | * |
dc.language | English | * |
dc.publisher | KARGER | * |
dc.subject | Neointimal hyperplasia | * |
dc.subject | Extracellular vesicles | * |
dc.subject | Endothelium | * |
dc.subject | Transforming growth factor-beta | * |
dc.subject | Vascular smooth muscle cells | * |
dc.title | Indoxyl Sulfate-Induced Extracellular Vesicles Released from Endothelial Cells Stimulate Vascular Smooth Muscle Cell Proliferation by Inducing Transforming Growth Factor-Beta Production | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 56 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 129 | * |
dc.relation.lastpage | 138 | * |
dc.relation.journaltitle | JOURNAL OF VASCULAR RESEARCH | * |
dc.identifier.doi | 10.1159/000496796 | * |
dc.identifier.wosid | WOS:000496485200003 | * |
dc.author.google | Ryu, Jung-Hwa | * |
dc.author.google | Jeon, Eun-Young | * |
dc.author.google | Kim, Seung-Jung | * |
dc.contributor.scopusid | 김승정(8619054500) | * |
dc.date.modifydate | 20240419142141 | * |