Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김규보 | * |
dc.date.accessioned | 2019-11-22T16:30:04Z | - |
dc.date.available | 2019-11-22T16:30:04Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0250-7005 | * |
dc.identifier.issn | 1791-7530 | * |
dc.identifier.other | OAK-25729 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252155 | - |
dc.description.abstract | Background/Aim: The aim of the present study was to suggest potential mechanisms of epidermal growth factor (EGF)-induced cell death and radiosensitization in EGF receptor (EGFR)-overexpressing cancer cell lines. Materials and Methods: Two EGFR-overexpressing cancer cell lines (AMC-HN3, and A431), one EGFR-null cancer cell line (H520) and normal fibroblasts were cultured with 0.01-1000 nM of recombinant human EGF (rhEGF), and a clonogenic assay was performed. After culturing serum-starved cells with 10 nM rhEGF, the expression patterns of two apoptosis-associated proteins (cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP)) and the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway were measured using immunoblotting. The radiosensitizing effect of EGF was also evaluated with a clonogenic assay and phosphorylated H2AX (gamma H2AX) immunofluorescence. Results: In the clonogenic assay, the number of colonies was decreased in a dose-dependent manner in EGFR-overexpressing cancer cell lines. The expression of cleaved caspase-3 and cleaved PARP was significantly induced in EGFR-overexpressing cancer cell lines. As for the PI3K/AKT/mTOR signaling pathway, EGF paradoxically suppressed the expression of PI3K, AKT and mTOR in a time-dependent manner. As for the radiosensitizing effect, EGF enhanced the radiosensitivity of AMC-HN3 and A431 cells. Conclusion: rhEGF treatment induced cell death in the two EGFR-overexpressing cancer cell lines, and the mode of cell death was apoptosis. Moreover, cell death might be associated with the paradoxical suppression of PI3K/AKT/mTOR signaling pathway. rhEGF in combination with radiation augmented the radiation effect in EGFR-overexpressing cancer cell lines via inhibition of DNA damage repair. | * |
dc.language | English | * |
dc.publisher | INT INST ANTICANCER RESEARCH | * |
dc.subject | EGF | * |
dc.subject | EGFR-overexpressing cancer cell line | * |
dc.subject | apoptosis | * |
dc.subject | PI3K/AKT/mTOR signaling pathway | * |
dc.subject | radiosensitization | * |
dc.title | Epidermal Growth Factor-induced Cell Death and Radiosensitization in Epidermal Growth Factor Receptor-overexpressing Cancer Cell Lines | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 35 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 245 | * |
dc.relation.lastpage | 253 | * |
dc.relation.journaltitle | ANTICANCER RESEARCH | * |
dc.identifier.wosid | WOS:000347735300030 | * |
dc.author.google | Kim, Kyubo | * |
dc.author.google | Wu, Hong-Gyun | * |
dc.author.google | Jeon, Sang-Rok | * |
dc.contributor.scopusid | 김규보(8213302900) | * |
dc.date.modifydate | 20240222162403 | * |