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Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter
- Title
- Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter
- Authors
- Van Dung Pham; Somasundaram, Sivachandiran; Lee, Seung Hwan; Park, Si Jae; Hong, Soon Ho
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2016
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- ISSN
- 1367-5435
1476-5535
- Citation
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 43, no. 1, pp. 79 - 86
- Keywords
- Co-localization; GABA; Protein scaffold; Synthetic biology
- Publisher
- SPRINGER HEIDELBERG
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways.
- DOI
- 10.1007/s10295-015-1712-8
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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