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Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter

Title
Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter
Authors
Van Dung PhamSomasundaram, SivachandiranLee, Seung HwanPark, Si JaeHong, Soon Ho
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN
1367-5435JCR Link

1476-5535JCR Link
Citation
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 43, no. 1, pp. 79 - 86
Keywords
Co-localizationGABAProtein scaffoldSynthetic biology
Publisher
SPRINGER HEIDELBERG
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways.
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DOI
10.1007/s10295-015-1712-8
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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