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Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli
- Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli
- Chae, Cheol Gi; Kim, You Jin; Lee, Se Jin; Oh, Young Hoon; Yang, Jung Eun; Joo, Jeong Chan; Kang, Kyoung Hee; Jang, Young-Ah; Lee, Hyuk; Park, A-Reum; Song, Bong Keun; Lee, Sang Yup; Park, Si Jae
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING vol. 21, no. 1, pp. 169 - 174
- PHA; 2-hydroxyacid; lactate; 2-hydroxybutyrate; poly(2-hydroxybutyrate-co-lactate); recombinant E. coli
- KOREAN SOC BIOTECHNOLOGY &
- SCIE; SCOPUS; KCI
- Document Type
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- We have previously reported in vivo biosynthesis of polyhydroxyalkanoates containing 2-hydroxyacid monomers such as lactate and 2-hydroxybutyrate in recombinant Escherichia coli strains by the expression of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1 (Ps6-19)). Here, we report the biosynthesis of poly(2-hydroxybutyrate-co-lactate)[P(2HB-co-LA)] by direct fermentation of metabolically engineered E. coli strain. Among E. coli strains WL3110, XL1-Blue, and BL21(DE3), recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 produced P(76.4mol%2HB-co-23.6mol%LA) to the highest content of 88 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 2-hydroxybutyrate. When recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 was cultured in a chemically defined medium containing 20 g/L of glucose and varying concentration of sodium 2-hydroxybutyrate, 2HB monomer fraction in P(2HB-co-LA) increased proportional to the concentration of sodium 2-hydroxybutyrate added to the culture medium. P(2HB-co-LA)] could also be produced from glucose as a sole carbon source without sodium 2-hydroxybutyrate into the culture medium. Recombinant E. coli XL1-Blue strain expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis Il1403 panE gene, successfully produced P(23.5mol%2HB-co-76.5mol%LA)] to the polymer content of 19.4 wt% when it cultured in a chemically defined medium containing 20 g/L of glucose. The metabolic engineering strategy reported here should be useful for the production of novel copolymer P(2HB-co-LA)].
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