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Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii

Title
Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii
Authors
Oh, Young HoonEom, Gyeong TaeKang, Kyoung HeeJoo, Jeong ChanJang, Young-AhChoi, Jae WooSong, Bong KeunLee, Seung HwanPark, Si Jae
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
BIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN
1615-7591JCR Link

1615-7605JCR Link
Citation
BIOPROCESS AND BIOSYSTEMS ENGINEERING vol. 39, no. 4, pp. 555 - 563
Keywords
Clostridium beijerinckiiE. coli-Clostridia shuttle vectorGene-expression cassettesLipase2-Propanol
Publisher
SPRINGER
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Gene-expression cassettes for the construction of recombinant Clostridium beijerinckii were developed as potential tools for metabolic engineering of C. beijerinckii. Gene expression cassettes containing ColE1 origin and pAMB origin along with the erythromycin resistance gene were constructed, in which promoters from Escherichia coli, Lactococcus lactis, Ralstonia eutropha, C. acetobutylicum, and C. beijerinckii are examined as potential promoters in C. beijerinckii. Zymogram analysis of the cell extracts and comparison of lipase activities of the recombinant C. beijerinckii strains expressing Pseudomonas fluorescens tliA gene suggested that the tliA gene was functionally expressed by all the examined promoters with different expression level. Also, recombinant C. beijerinckii expressing C. beijerinckii secondary alcohol dehydrogenase by the constructed expression cassettes successfully produced 2-propanol from glucose. The best promoter for TliA expression was the R. eutropha phaP promoter while that for 2-propanol production was the putative C. beijerinckii pta promoter. Gene expression cassettes developed in this study may be useful tools for the construction of recombinant C. beijerinckii strains as host strains for the valuable chemicals and fuels from renewable resources.
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DOI
10.1007/s00449-016-1537-5
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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