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dc.contributor.author박시재-
dc.date.accessioned2019-11-19T16:30:47Z-
dc.date.available2019-11-19T16:30:47Z-
dc.date.issued2016-
dc.identifier.issn1087-0156-
dc.identifier.issn1546-1696-
dc.identifier.otherOAK-25864-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/252005-
dc.description.abstractPoly(lactate-co-glycolate) (PLGA) is a widely used biodegradable and biocompatible synthetic polymer. Here we report one-step fermentative production of PLGA in engineered Escherichia coli harboring an evolved polyhydroxyalkanoate (PHA) synthase that polymerizes D-lactyl-CoA and glycolyl-CoA into PLGA. Introduction of the Dahms pathway enables production of glycolate from xylose. Deletion of ptsG enables simultaneous utilization of glucose and xylose. An evolved propionyl-CoA transferase converts D-lactate and glycolate to D-lactyl-CoA and glycolyl-CoA, respectively. Deletion of adhE, frdB, pflB and poxB prevents by-product formation. We also demonstrate modulation of the monomer fractions in PLGA by overexpressing ldhA and deleting dld to increase the proportion of D-lactate or by deleting aceB, glcB, glcD, glcE, glcF and glcG to increase the proportion of glycolate. Incorporation of 2-hydroxybutyrate is prevented by deleting ilvA or feeding strains with l-isoleucine. The utility of our approach for generating diverse forms of PLGA is shown by the production of copolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate or 2-hydroxyisovalerate.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleOne-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume34-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage435-
dc.relation.lastpage+-
dc.relation.journaltitleNATURE BIOTECHNOLOGY-
dc.identifier.doi10.1038/nbt.3485-
dc.identifier.wosidWOS:000373616100026-
dc.author.googleChoi, So Young-
dc.author.googlePark, Si Jae-
dc.author.googleKim, Won Jun-
dc.author.googleYang, Jung Eun-
dc.author.googleLee, Hyuk-
dc.author.googleShin, Jihoon-
dc.author.googleLee, Sang Yup-
dc.contributor.scopusid박시재(57191670770)-
dc.date.modifydate20210929141221-
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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