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One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli

Title
One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli
Authors
Choi, So YoungPark, Si JaeKim, Won JunYang, Jung EunLee, HyukShin, JihoonLee, Sang Yup
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
NATURE BIOTECHNOLOGY
ISSN
1087-0156JCR Link

1546-1696JCR Link
Citation
NATURE BIOTECHNOLOGY vol. 34, no. 4, pp. 435 - +
Publisher
NATURE PUBLISHING GROUP
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Poly(lactate-co-glycolate) (PLGA) is a widely used biodegradable and biocompatible synthetic polymer. Here we report one-step fermentative production of PLGA in engineered Escherichia coli harboring an evolved polyhydroxyalkanoate (PHA) synthase that polymerizes D-lactyl-CoA and glycolyl-CoA into PLGA. Introduction of the Dahms pathway enables production of glycolate from xylose. Deletion of ptsG enables simultaneous utilization of glucose and xylose. An evolved propionyl-CoA transferase converts D-lactate and glycolate to D-lactyl-CoA and glycolyl-CoA, respectively. Deletion of adhE, frdB, pflB and poxB prevents by-product formation. We also demonstrate modulation of the monomer fractions in PLGA by overexpressing ldhA and deleting dld to increase the proportion of D-lactate or by deleting aceB, glcB, glcD, glcE, glcF and glcG to increase the proportion of glycolate. Incorporation of 2-hydroxybutyrate is prevented by deleting ilvA or feeding strains with l-isoleucine. The utility of our approach for generating diverse forms of PLGA is shown by the production of copolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate or 2-hydroxyisovalerate.
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DOI
10.1038/nbt.3485
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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