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Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli

Title
Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli
Authors
Van Dung PhamSomasundaram, SivachandiranLee, Seung HwanPark, Si JaeHong, Soon Ho
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN
0273-2289JCR Link

1559-0291JCR Link
Citation
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY vol. 178, no. 7, pp. 1315 - 1324
Keywords
Gamma-aminobutyric acidGlucoseMetabolic pathwayRecombinant DNASynthetic scaffold
Publisher
HUMANA PRESS INC
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
In general, gamma-aminobutyric acid (GABA) pathway involves the decarboxylation of glutamate, which is produced from sugar by Corynebacterium fermentation. GABA can be used for the production of pharmaceuticals and functional foods. Due to the increasing demand of GABA, it is essential to create an effective alternative pathway for the GABA production. In this study, Escherichia coli were engineered to produce GABA from glucose via GABA shunt, which consists of succinate dehydrogenase, succinate-semialdehyde dehydrogenase, and GABA aminotransferase. The three enzymes were physically attached to each other through a synthetic scaffold, and the Krebs cycle flux was redirected to the GABA pathway. By introduction of synthetic scaffold, 0.75 g/l of GABA was produced from 10 g/l of glucose at 30 A degrees C and pH 6.5. The inactivation of competing metabolic pathways provided 15.4 % increase in the final GABA concentration.
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DOI
10.1007/s12010-015-1948-9
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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