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Gamma-aminobutyric acid production through GABA shunt by synthetic scaffolds introduction in recombinant Escherichia coli
- Title
- Gamma-aminobutyric acid production through GABA shunt by synthetic scaffolds introduction in recombinant Escherichia coli
- Authors
- Van Dung Pham; Somasundaram, Sivachandiran; Lee, Seung Hwan; Park, Si Jae; Hong, Soon Ho
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2016
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- ISSN
- 1226-8372
1976-3816
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING vol. 21, no. 2, pp. 261 - 267
- Keywords
- carbon flux; GABA shunt; glucose; metabolites; recombinant DNA; synthetic scaffold
- Publisher
- KOREAN SOC BIOTECHNOLOGY &
BIOENGINEERING
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Nylon 4 is a biodegradable polymer which can be produced from the monomer of pyrrolidone. Gammaaminobutyric acid (GABA) is a precursor of pyrrolidone used for the production of bioplastics. In this study, Escherichia coli were engineered to produce gammaaminobutyric acid from glucose via an alternative novel pathway by the introduction of synthetic scaffolds. The GABA pathway constructed contained succinate dehydrogenase, succinate-semialdehyde dehydrogenase and GABA aminotransferase to redirect the Krebs cycle flux to GABA production. By introduction of a synthetic scaffold, production of 0.64 g/L GABA was achieved at 30A degrees C and pH 6.5. Final GABA concentration was increased by 11.3% via the inactivation of competing pathways, and higher initial glucose concentration led to the enhanced final GABA concentration of 1.01 g/L.
- DOI
- 10.1007/s12257-015-0783-8
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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