Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:38Z | - |
dc.date.available | 2019-11-19T16:30:38Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.issn | 1738-8872 | * |
dc.identifier.other | OAK-25892 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251977 | - |
dc.description.abstract | Gamma-aminobutyric acid (GABA) is a non-protein amino acid, which is an important inhibitor of neurotransmission in the human brain. GABA is also used as the precursor of biopolymer Nylon-4 production. In this study, the carbon flux from the tricarboxylic acid cycle was directed to the GABA shunt pathway for the production of GABA from glucose. The GABA shunt enzymes succinate-semialdehyde dehydrogenase (GabD) and GABA aminotransferase (GabT) were co-localized along with the GABA transporter (GadC) by using a synthetic scaffold complex. The co-localized enzyme scaffold complex produced 0.71 g/l of GABA from 10 g/l of glucose. Inactivation of competing metabolic pathways in mutant E. coli strains XBM1 and XBM6 increased GABA production 13% to reach 0.80 g/l GABA by the enzymes co-localized and expressed in the mutant strains. The recombinant E. coli system developed in this study demonstrated the possibility of the pathway of the GABA shunt as a novel GABA production pathway. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC MICROBIOLOGY & | * |
dc.publisher | BIOTECHNOLOGY | * |
dc.subject | Gamma-aminobutyric acid | * |
dc.subject | glucose | * |
dc.subject | co-localization | * |
dc.subject | scaffold complex | * |
dc.subject | recombinant DNA | * |
dc.subject | carbon flux | * |
dc.title | Co-Localization of GABA Shunt Enzymes for the Efficient Production of Gamma-Aminobutyric Acid via GABA Shunt Pathway in Escherichia coli | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 26 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 710 | * |
dc.relation.lastpage | 716 | * |
dc.relation.journaltitle | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | * |
dc.identifier.doi | 10.4014/jmb.1511.11037 | * |
dc.identifier.wosid | WOS:000378354800009 | * |
dc.author.google | Van Dung Pham | * |
dc.author.google | Somasundaram, Sivachandiran | * |
dc.author.google | Park, Si Jae | * |
dc.author.google | Lee, Seung Hwan | * |
dc.author.google | Hong, Soon Ho | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |